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This Antibody Can be Used for Immunohistochemistry and Western Blot Study
(1)
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Cell Signaling Technology
July 17, 2015
I do cloning for animal experiments to use as liver cancer models and thus use a variety of molecular biology reagents including antibodies,...
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Review of DAB substrate kit
(1)
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Abcam
July 17, 2015
To perform immunohistochemical staining, tissue sections were dewaxed with xylene and rehydrated through gradient ethanol into water. For ...
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Good invariant chain intracellular staining
(1)
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BD Biosciences
July 17, 2015
We used this antibody to investigate the functionality of the MHC II antigen presentation pathway in murine cells using flow cytometry. This...
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In vitro culture of primary human keratinocytes in epilife media supplemented with HKGS
(1)
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Thermo Fisher Scientific
July 16, 2015
Human dermal keratinocytes isolated from dermis of human foreskin or other dermal tissue is cultured in vitro with epilife medium M-EPI-500-...
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Low cost anti-His antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 16, 2015
We analyzed samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according ...
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Antibody Used for Western Blot
(1)
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July 16, 2015
We performed many Western blots using this antibody. It is a mouse Monoclonal Antibody that detects recombinant proteins containing V5 tag.
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The DeNovix DS-11 Spectrophotometer
(1)
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DeNovix Inc.
July 16, 2015
Our group does a lot of cloning using restriction enzymes. Typically, we have small volumes of the materials along with low quantities after...
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Competent Cells Without Plasmid Methylation Problems
(1)
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New England Biolabs
July 16, 2015
I use these competent cells when I need to produce DNA plasmid that is free of dam or dcm methylation. This is important when I need to ...
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Ward's Science Provides Excellent Histology Services
(1)
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July 15, 2015
I brought fixed tissues (mouse kidneys and eyes) to Ward's Scientific in tissue cassettes in 70% ethanol. The tissues were embedded in ...
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Mouse Holder for Injection
(1)
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Braintree Scientific
July 15, 2015
We used the holder for mouse hydrodynamic tail injection. The design is very convenient for mouse fixation because it has 3 stickers to fix ...
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Review of Anti-GAPDH antibody
(1)
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Abcam
July 15, 2015
To perform Western Blot analysis, the cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM ...
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Western blot antibody for PTEN from Santa Cruz Biotechnology
(1)
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Santa Cruz Biotechnology, Inc.
July 15, 2015
I used this antibody to detect PTEN expression in mouse T cells. I preferrred this because it has multiple species reactivity for human, ...
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Extremely reliable anti-His antibody
(1)
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Cytiva Life Sciences
July 15, 2015
His tagged proteins can be easily detected using this antibody, regardless of recombinant, transiently or stably transformed in plants.
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Anti-Human/Mouse/Rat OX40L antibody for Western blot
(1)
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Thermo Fisher Scientific
July 14, 2015
I used to this antibody to detect my recombinant human OX40L expressed in 293T cells. I prefer this antibody because it has species re-...
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Western blot antibody for PTEN from R&D systems
(1)
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R&D Systems, a Bio-Techne Brand
July 14, 2015
I used this antibody to detect mouse PTEN by Western blot. Earlier, I had issues with anti-PTEN antibody from SCBT so I tried this antibody ...
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Great anti-Ubiquitin antibody for total cell lysate
(1)
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Santa Cruz Biotechnology, Inc.
July 14, 2015
We analyze sample of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to ...
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Efficient Electroporation with NEON Transfection System
(1)
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July 14, 2015
I use the Neon system to electroporate cells that are usually difficult to transfect with more classic reagents such as lipofectamine. Here ...
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V450 Rat Anti-Mouse CD8a for FACS
(1)
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BD Biosciences
July 14, 2015
The 53-6.7 monoclonal antibody from BD Biosciences specifically binds to the 38 kDa a and 34 kDa a' chains of the CD8 differentiation ...
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Review for Anti-beta Actin antibody
(1)
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Abcam
July 14, 2015
To perform Western Blot analysis, the cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM ...
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Chicken anti-HA epitope tag
(1)
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Abcam
July 13, 2015
This antibody was used to perform immunofluorescence microscopy on HeLa cells transfected with an HA-containing construct. Ab9111 was used ...
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Excellent knockdown of intracellular trafficking proteins in human cell lines
(1)
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Thermo Fisher Scientific
July 13, 2015
Our research explores the interaction of drug and gene delivery vehicles with intracellular trafficking processes such as those associated ...
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Review of Anti-alpha Tubulin antibody (ab4074)
(1)
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Abcam
July 13, 2015
To perform Western Blot Analysis, cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1...
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Quality pH Meter
(1)
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Thermo Fisher Scientific
July 13, 2015
I use this to pH test a variety of solutions I make in the lab. I like this pH meter because it is easy to use and calibrate. Additionally, ...
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Good A7L6 Antibody
(1)
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Thermo Fisher Scientific
July 13, 2015
We wanted to see where in the human nasal sinuses we could detect heparan sulfate.
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A not-so-useful spleen-based antibody for immunofluorescence
(1)
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Bio-Rad
July 10, 2015
Our research looks at immune cells such as macrophages, lymphocytes, and dendritic cells. One of our experiments required us to identify ...
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Very Reliable, Easy-to-use Polymerase for Difficult DNA Templates
(1)
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Bioline
July 10, 2015
I was trying to perform PCR on a difficult, GC-rich genomic template. I tried MyFi Mix because it is ready to use, compatible with TA ...
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Good Protein Ladder
(1)
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Bio-Rad
July 10, 2015
I use this product when running protein gels. I like this product because it is already mixed so I just have to add the solution to the gel ...
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Anti-DHFR for Westerns and IPs
(1)
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Santa Cruz Biotechnology, Inc.
July 10, 2015
Mouse dehydrofolate reductase (DHFR) can be easily detected using this antibody. We analyzed samples of crude Arabidopsis thaliana (mouse-...
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Very Good Anti-mouse CD8 antibody
(1)
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July 10, 2015
Balb/C mice were sacrificed and single cell suspensions prepared from the spleens were used for flow cytometric analysis. This antibody was ...
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Great antibody for detecting mouse CD4 expression
(1)
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BD Biosciences
July 09, 2015
Balb/C mice were sacrificed and isolated splencoytes were used for flow cytometric analysis . Cells were stained with anti-CD4 antibody (...
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Sca-1 Antibody
(1)
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BioLegend
July 09, 2015
We use this to stain Sca-1 in the bone marrow for flow cytometric analysis.
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Phospho - PI3KD
(1)
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Biorbyt
July 09, 2015
I work on trying to understand the signaling mechanism of a surface receptor expressed on eosinophils and PI3KD is a potential signaling ...
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Excellent antibody to detect phospho-Pak in human cells
(1)
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Abcam
July 09, 2015
Our experiment was planned to check the effect of Pak1 knockdown in VEGFA induced Pak1 phosphorylation in Human retinal endothelial cells. ...
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Good gel electrophoresis chamber
(1)
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Fotodyne Inc.
July 09, 2015
I use this electrophoresis chamber when running DNA agarose gels. I like this chamber because it is compact so it does not take up a lot of ...
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Good antibody to detect Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
(1)
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Cell Signaling Technology
July 08, 2015
We tested the time course effect of FGF2 on PRK1/PRK2 phosphorylation in human retinal endothelial cells. Cell Signaling antibody worked ...
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Great phospho-STAT3 antibody
(1)
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Cell Signaling Technology
July 08, 2015
I use this antibody to detect the levels of STAT3 phosphorylated at tyrosine 705 to see the affects different signaling pathway inhibitors ...
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Reliable rabbit anti-GFP
(1)
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Santa Cruz Biotechnology, Inc.
July 08, 2015
We use this Green Fluorescent Protein (GFP) antibody to target our primary antibody. It needs some optimization and dilution steps to ...
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Dual-Luciferase Reporter Gene Assay
(1)
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July 08, 2015
I do work with liver pathology and have a strong background and experience in innovative liver tumor models, molecular biology techniques ...
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Reliable mouse anti-GFP
(1)
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July 08, 2015
Green fluorescent protein (GFP) can be easily detected using this antibody.
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Great benchtop centrifuge
(1)
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Eppendorf
July 07, 2015
I centrifuge a lot solutions in the lab for a variety of applications. I like using this centrifuge because it is compact so it easily fits ...
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Commercial RIN4 antibody for recombinant protein
(1)
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Santa Cruz Biotechnology, Inc.
July 07, 2015
This RIN4 antibody recognizes the peptide epitope from Arabidopsis; it works for in vitro translated protein but not for plant extracts.
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Site-direct mutagenesis to study role of Ser/Thr/Tyr phosphorylation or Acetylation
(1)
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Agilent Technologies
July 07, 2015
I used this kit to make a mutation of Ser to Ala amino acids in my expression construct. It is very easy and fast to do with this kit.
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Detection of Phospho p38 MAPK
(1)
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Cell Signaling Technology
July 07, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here in ...
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CD45 Antibody
(1)
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BioLegend
July 07, 2015
This antibody is used to stain CD45 for flow cytometry.
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Use of Miltenyi Biotec QuadroMacs Seperation Unit in the negative selection of CD4+ T cells
(1)
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Miltenyi Biotec
July 06, 2015
The Quadromacs Seperation unit is used to seperate CD4+ T cells from lymph nodes and spleens of immunized mice.
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Detection of Phospho ERK
(1)
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Cell Signaling Technology
July 06, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here in ...
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Good DNA Loading Dye
(1)
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Promega
July 06, 2015
I use this loading dye when I am running DNA samples on agarose gels. I like this product because it gives multiple bands to help me know ...
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Fixable Live/Dead Stain
(1)
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Thermo Fisher Scientific
July 06, 2015
We use this stain on many cell types to distinguish live cells and dead cells for flow cytometry.
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Commercial RIN4 antibody for plants
(1)
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Santa Cruz Biotechnology, Inc.
July 06, 2015
This RIN4 antibody recognizes the peptide epitope from Arabidopsis; it works for plant extracts but not for in vitro translated protein.
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Cheaper Alternative to Sigma REDExtract-N-Amp
(1)
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Bioline
July 02, 2015
We use this product for genotyping, and it works really well. We switched because it was a lot cheaper than the Sigma brand one, but ...
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