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Date Published
Obtaining Retroviruses for Superior Transduction of Human Cells
(1)
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Thermo Fisher Scientific
July 02, 2015
Retroviruses are capable of highly efficient, stable transfection of human cells. In order to obtain stably transfected cell lines for our ...
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BD CompBeads Anti-Rat and Anti-Hamster
(1)
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BD Biosciences
July 02, 2015
We were assessing Aryl hydrocarbon receptor activation in bone marrow derived dendritic cells. Precursor cells were isolated with Lympholyte...
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Fantastic for measuring epithelial barrier function
(1)
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World Precision Instruments
July 02, 2015
I got this voltmeter about 3 years ago, and it still works great. I measure barrier function of human airway epithelial cells cultured at ...
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BD CompBeads Negative Control
(1)
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BD Biosciences
July 02, 2015
We were assessing Aryl hydrocarbon receptor activation in bone marrow derived dendritic cells. Precursor cells were isolated with Lympholyte...
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Excellent recognition of CD68+ macrophages in various tissues of the mice
(1)
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Bio-Rad
July 01, 2015
Macrophages can be identified in tissue cross-sections via the CD68 intracellular marker. We used this product along with the use of Cell ...
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IDH1 Screening in Disease Conditions Through Western Blotting
(1)
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July 01, 2015
We were looking for the role of IDH1 in different types of cancer tumour metabolism using techniques like Western blotting. This product was...
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Rabbit alexa488 as an secondary for immunofluorescence
(1)
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Abcam
July 01, 2015
We used this antibody to check protein expression changes in neuronal cells using immunofluorescence.
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Mouse tail Direct PCR Kit
(1)
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July 01, 2015
Our aim is to maintain the breeding colony of our laboratory mice which is a KO mice. Specifically, our goal is to screen the KO, WT and ...
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Good anti-Mouse CD3 antibody
(1)
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BD Biosciences
July 01, 2015
The aim of this experiment was to identify the percentage of CD3 positive T cells in the spleen of control and experimental groups. For ...
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Anti-Galpha 11 Antibody for Western blot and Immunoprecipitation
(1)
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Santa Cruz Biotechnology, Inc.
June 30, 2015
This antibody was used to study the role of chemokines on GPCR receptor activation. I used this antibody for an Immunoprecipitation (IP) ...
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FGFR Antibody to Locate the Presence of FGFR1
(1)
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Novus Biologicals, a Bio-Techne Brand
June 30, 2015
Our laboratory selects aptamers for various cell receptors. To test the specificity of aptamer binding to white blood cells or transfected ...
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FASLG Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
June 30, 2015
Hela cell lysate of 25 ug protein was loaded onto SDS gel (4%-12%) and MES was used for electrophoresis. Major bands appeared at ~90 kDa and...
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High background level
(1)
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Santa Cruz Biotechnology, Inc.
June 30, 2015
We used this antibody to evaluate whether cells expressed an increased level of MHC II molecules containing the CLIP peptide on their ...
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Flag tag protein over expression in breast cancer analyzed through Western blot
(1)
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June 30, 2015
RunX1 is an transcription factor which regulates differentiation of haematopoietic stem cells. This protein has an association with ...
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Good occludin antibody
(1)
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Thermo Fisher Scientific
June 29, 2015
This antibody works well on differentiated human airway epithelial cells. We use at a 1:200 dilution, and store it in small aliquots at -20.
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Detection of Phospho p65 using flow
(1)
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Abcam
June 29, 2015
Our long term goal is to understand the process of tumorigenesis in colon cancer cells. In this experiment, we treated colon cancer cells ...
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Detection of NCR1 in NK cells
(1)
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Abcam
June 29, 2015
Our long term goal is to understand the tumorigenesis process in different cancer types. Immune component does play a major role in the ...
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Use of Evans Blue to monitor Blood Brain Barrier Permeability
(1)
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June 29, 2015
We wanted to analyse blood brain barrier permeability in a neurologically diseased mouse model. There are many ways to analyse this but we ...
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Cytotoxicity Detection kit, Roche applied science
(1)
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Roche
June 29, 2015
I have used this kit several times, and it is really simple and straight forward. The only thing that is not simple is the instruction ...
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Convenient, easy to use
(1)
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June 26, 2015
Our lab uses PCR for a number of purposes (cloning, mutation confirmation, etc), mostly with E. coli and Staphylococcal DNA. We have found ...
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Great plasmid prep kit
(1)
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June 26, 2015
Our lab does quite a bit of cloning and thus do a lot of DNA preps. We generally use the Zippy kit for E. coli and it works wonderfully. In ...
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ELISpot feasibility
(1)
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June 26, 2015
Overall the product has proven to be effective. Although, the description lacks the identification of required reagents that must be ...
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Confocal Microscope
(1)
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Leica Microsystems
June 26, 2015
My project involves elucidating the roles of Eps15 Homology Domain containing protein 4 (EHD4) in the kidney. EHD4 is a member of the EHD ...
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MQ10 Flow Cytometer
(1)
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Miltenyi Biotec
June 26, 2015
We are examining the role of innate and adaptive immune cells in disease states in large population studies. We needed a machine capable of...
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Benchtop PCR plate centrifuge from Labnet
(1)
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Corning Life Sciences
June 25, 2015
This is a convenient and compact benchtop centrifuge for PCR plates.
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Treg Isolation
(1)
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Miltenyi Biotec
June 25, 2015
This Kit is very useful for isolating CD4+CD25+ Tregs from LN and Spleen, in order to culture Tregs, using them for RT PCR or for adoptive ...
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Use of Isotemp 205 waterbath
(1)
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Fisher Scientific
June 25, 2015
This Fisher Scientific Isotemp 205 waterbath was used in the heat inactivation of FBS.
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BACH2 polyclonal antibody for IHC
(1)
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June 25, 2015
BACH2 is known to play a role in B cell maturation. I have used the BACH2 antibody to assess BACH2 protein expression in high grade B-cell ...
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GAPDH Western Blot detection
(1)
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Cell Signaling Technology
June 25, 2015
Our labs longstanding goal is to understand the mistranslocation of cancer specific proteins. Here in this study we are evaluating different...
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Clean and specific PIAS1 antibody
(1)
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Cell Signaling Technology
June 24, 2015
PIAS1, an E3-type SUMO ligase tethering SUMO moiety to substrate proteins, is crucial for protein functions and transcriptional coregulation...
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Bio Rad SYBR Green Supermix
(1)
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Bio-Rad
June 24, 2015
For these experiments we were assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells were ...
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Classic reagent for RNA/DNA/protein isolation from cells and tissues
(1)
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June 24, 2015
We use TRIzol to extract RNA from various cells and tissues for PCR amplification. The general aim is to study changes in gene expression. ...
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Rabbit anti-V5 immunofluorescence microscopy
(1)
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Cell Signaling Technology
June 24, 2015
This antibody was used to perform immunofluorescence microscopy on HeLa cells transfected with a V5 containing construct. Several anti-V5 ...
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Use of Rad Source irradiator in the irradiation of splenocytes
(1)
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June 24, 2015
The Rad Source RS-2000 Biological System is used in the irradiation of a single cell suspension of splenocytes.
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Used for H&E staining
(1)
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Fisher BioReagents
June 24, 2015
This is a very good kit for H&E staining, the nuclear staining is strong enough to separated and the color is very nice, especially for the ...
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Good Miniprep Kit
(1)
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June 23, 2015
I use this kit when I need to make relatively small amounts of DNA to use for restriction digestion, colony PCR, etc. I like this kit ...
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Good kit for transfecting cells
(1)
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June 23, 2015
I use this kit to transfect HEK293T cells in the lab when making virus. I like this kit because I can achieve 90+% transfection with a ...
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RNeasy Plus Mini Kit (250)
(1)
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QIAGEN - Sample to Insight
June 23, 2015
For these experiments we were assessing Aryl hydrocarbon receptor activation in bone marrow derived dendritic cells. Precursor cells were ...
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Robust and Efficient Heating Block for traditional PCR reactions
(1)
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Bio-Rad
June 23, 2015
In the age of digital and real-time PCR, traditional PCR may have been cornered to the backseat. However, for isolation of large amounts of ...
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Total caspase 3 detection
(1)
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Cell Signaling Technology
June 23, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here in ...
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Good plasmid isolation kit
(1)
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Omega Engineering Inc.
June 22, 2015
I use this kit when I need to isolate plasmid DNA in the lab for cloning purposes. I like this kit because I never have trouble using the ...
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Plasmid maxiprep kit especially good for in vivo experiments
(1)
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June 22, 2015
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit could be used to extract plasmid in maxi in a short amount of time, it just takes about ...
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Easy and high titer production of retroviruses from a producer cell line
(1)
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June 22, 2015
Generation of retroviral vectors from a producer cell needs to be reliable for downstream applications for therapeutics development. We used...
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Valuable Isotype control for flow cytometry of peritoneal macrophages to confirm F4/80 staining
(1)
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June 22, 2015
Peritoneal macrophages can be easily sorted and analyzed via flow cytometry using the pan-macrophage marker - F4/80. However, to confirm and...
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Good RAF1 antibody for Western Blot
(1)
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MilliporeSigma
June 22, 2015
I was looking at RAF1 expression in iPSCs to study RAF1 function in human iPSC-derived cardiomyocytes. I used this product to detect ...
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Good quality L-broth with low price
(1)
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USBiological
June 19, 2015
We use this particular L-Broth (Luria Broth) because the LB powder is same quality as other companys' products, but the price has a huge ...
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Total ROS detection in eosinophils
(1)
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Enzo Life Sciences, Inc.
June 19, 2015
I work on trying to determine the source of ROS production in eosinophils following the engagement of a eosinophil specific surface receptor...
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Analysis of T-cell Central Memory Population
(1)
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BioLegend
June 19, 2015
A small fraction of Naive T cells upon primary exposure to foreign or self antigens differentiate to form a population called T central ...
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Quick and Easy Mix for quantifying multiple samples for retroviral titer
(1)
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June 19, 2015
Retroviruses are critical in most therapeutic and gene transduction experiments. To optimize their performance, quantifying their titer post...
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Fast Plasmid Midiprep Kit
(1)
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June 19, 2015
We use this kit as it is very convenient for doing midi-preparation of plasmid from bacterial culture, the whole process takes about 30 ...
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Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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