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Date Published
Review for Anti-14-3-3 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
August 07, 2015
Cells were washed twice in PBS and harvested with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-...
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Time Course Effect of MCP-1 Induced Phosho p70S6 Kinase in HASMS
(1)
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Cell Signaling Technology
August 07, 2015
The aim of this study is to check the MCP-1 induced pp70S6K role in HASMS. These p70S6 kinase is involved in p70 S6 is a mitogen activated ...
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Superb Antibody to Study IDH2 Related Disease Mechanism
(1)
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August 07, 2015
We used this antibody to study IDH2 mutation which has a role in Glioblastoma such as grade 3 and grade 4 tumours.
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Detection of EpCAM in Cancer Cells
(1)
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Abcam
August 07, 2015
Our longstanding goal is to understand the role of biomarkers in different cell lines. Here we test the detection of EpCAM, an epithelial ...
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FBRS Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
August 07, 2015
Our current project is all about identification of affected proteins and their levels upon specific treatment of mammalian (HeLa) cells.
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Review for Anti-PKD2 Antibody
(1)
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Abcam
August 06, 2015
Cells were washed twice in PBS and harvested with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-...
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Review for Anti-SSH2 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
August 06, 2015
Cells were washed twice in PBS and harvested with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-...
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Best Result from Isolectin GS-IB4, Alexa Fluor 488 Conjugate
(1)
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Thermo Fisher Scientific
August 06, 2015
Isolectin GS-IB4 , Alexa Fluor 488 Conjugate from Life Technologies was used to detect mouse retinal vasculature. This product showed ...
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Pharmacologically Active In Vivo Use of Vorinostat
(1)
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Selleck Chemicals
August 06, 2015
Vorinostat is being used as a HIV-1 latency reversing agent in human trials. I wished to investigate the effect of this HDAC inhibitor on ...
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Antibody to Study Bcat1 Signaling Mechanism in Various Cancers
(1)
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Cell Signaling Technology
August 06, 2015
We used this antibody to study the BCAT1 signaling mechanism in various cancers like AML, breast cancer, and Glioblastoma.
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Good Antibody from Santa Cruz Biotechnology to Detect VEGFD
(1)
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Santa Cruz Biotechnology, Inc.
August 05, 2015
Experiment was planned to test the time course effect of growth factor on mammalian cell line. Cells were cultured, quiesced and treated ...
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Poor Quality Plasmid Prep and No Solution Offered
(1)
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August 05, 2015
I sent the DNA for three plasmids (pcDNA vectors) off to plasmid.com for transformation, amplification and purification (maxi size prep for ...
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Review for Anti-PKD3 Antibody
(1)
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Cell Signaling Technology
August 05, 2015
Cells were washed twice in PBS and harvested with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-...
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A Marker for Human M1 Macrophages
(1)
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BioLegend
August 05, 2015
It is often very difficult to distinguish between human M1 and M2 macrophages. This is because there is no universal marker that ...
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Phalloidin, Rhodamine Conjugate
(1)
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Biotium, Inc.
August 05, 2015
This product was used to visualize F-actin stress fiber formation in mammalian cells after treatment with chemotactic agents. After ...
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C Tubes & GentleMACS Dissociator for Tumor Dissociation
(1)
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Miltenyi Biotec
August 05, 2015
Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation using the gentleMACS™ Dissociator, and are very helpful in doing ...
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Review for Rabbit Anti–Phospho-IkB-Alpha Antibody
(1)
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Cell Signaling Technology
August 04, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Good Quality Large-Scale Plasmid Isolation Kit
(1)
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QIAGEN - Sample to Insight
August 04, 2015
EndoFree Plasmid Maxi Kit from QIAGEN was used to isolate 7.5kb mammalian overexpression plasmid. The purpose of plasmid purification was to...
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RNA Isolation from Cell Samples Using RNeasy Mini Kit
(1)
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QIAGEN - Sample to Insight
August 04, 2015
This product was used to isolate RNA from Neuro2a cells with different experimental conditions and to check the gene of interest (BDNF) ...
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Detection of ASPN in Cancer Cells
(1)
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Abcam
August 04, 2015
Here we test the detection of asporin protein in different osteosarcoma cell lines using Western blotting and from the data it can be seen ...
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Excellent Kit to Determine IL-23 Levels in Serum
(1)
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BioLegend
August 04, 2015
We use this kit on a regular basis to determine the levels of IL-23 in mice injected with various types of minicircle DNA. Because the ...
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Review for Rabbit Anti–IkB-a Antibody
(1)
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Cell Signaling Technology
August 03, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Detection of ASPN in Cancer Cells
(1)
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Abcam
August 03, 2015
Our longstanding goal is to understand the role of proteins in cancer progression. Here we detect the expression of ASPN protein in ...
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Western Blot Stripping Buffer Works Great and Can Re-Use
(1)
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August 03, 2015
For Western blot analysis, we strip the membranes so we can identify two proteins that are at very similar molecular weights.
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Effect of Chemotactic Agent on Orai1 Expression
(1)
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Santa Cruz Biotechnology, Inc.
August 03, 2015
This antibody was used in a study of chemotactic agent effect on Orai1 expression in mammalian cell line. I checked the study state levels ...
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Excellent Antibody for IHC-Fr
(1)
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Abcam
August 03, 2015
We wanted to detect complement activation in nasal tissue. Therefore we investigated if C4d was deposited in nasal tissue.
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Review of Mouse Anti-ADAR1 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 31, 2015
Protein extract from mouse MEFs was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied ...
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Easy for Routine Genotyping
(1)
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Biotium, Inc.
July 31, 2015
We genotype a lot of mice and this works well with agarose gels. Bands are easily detectable and it's easy to increase or decrease the ...
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Review for Human TREM-1 ELISA Kit
(1)
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R&D Systems, a Bio-Techne Brand
July 31, 2015
The general aim of my research was to investigate the expression of TREM-1 in urological cancers. I used this kit specifically to measure ...
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THE™ V5 Antibody
(1)
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July 31, 2015
This antibody was used to perform immunofluorescence microscopy on SW480 cells transfected with a V5-containing construct. This antibody was...
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Facilitated RNA extraction
(1)
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QIAGEN - Sample to Insight
July 31, 2015
Extracting RNA from cell lines or from tissue specimens has the historic connotation of being an arduous process. This kit, from Qiagen, ...
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Beware... if you're shopping for Autoclaves!
(1)
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July 30, 2015
The Getinge Model 522LS Sterilizer/Autoclave is a cost-competitive instrument that appealed to my department. Unfortunately, in the 5 years...
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Ki-67 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
July 30, 2015
The Ki67 antibody was used for IHC in the prostate to validate that the cancer cells are actively proliferating. Ki67, when this occurs, is...
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Immunocytochemistry of a Her2 Variant
(1)
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R&D Systems, a Bio-Techne Brand
July 30, 2015
We are studying Her2 expression in a number of cancer cell lines that may express Her2 variants. We selected this antibody because it ...
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Review for Mouse Anti-ß-Actin Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 30, 2015
Protein extract from MEFs was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Review for Rabbit Anti–Phospho-IRF3 Antibody
(1)
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Cell Signaling Technology
July 30, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Ninja TAQ Found be a Very Robust and Sensitive Enzyme for Amplification
(1)
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Pharmozyme
July 29, 2015
This enzyme was found to be better than any other commercially available TAQ polymerase. It works well for 200bp product amplification. Also...
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Good Kit for Cleaning the Reaction Components
(1)
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QIAGEN - Sample to Insight
July 29, 2015
We use this kit for cloning purpose. The amplified PCR product is purified from total reaction mixture. The purified PCR product is directly...
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Detection of PGP in Colon Cancer Cells
(1)
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Abcam
July 29, 2015
One of the known methods of developing tumor resistance is via the expression of PGP. Here we test if the antibody can detect PGP in colon ...
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Review for Rabbit Anti-IRF3 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 29, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Real Time PCR with High Efficiency
(1)
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Analytik Jena
July 29, 2015
We are interested in minimum residual disease also called MRD. We used the qTOWER for our studies on MRD and we gained 2 points for Ct ...
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Pierce ECL Plus Western Blotting Substrate
(1)
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July 28, 2015
This product was used to check the protein (transcription factors pCREB etc) expression changes in neuronal activity. This product generates...
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Pharmacologically Active In Vivo Use of Romidepsin
(1)
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Selleck Chemicals
July 28, 2015
To use HDAC inhibitors in an attempt to recapitulate HIV-1 latency reversion, but in mice. Romidepesin was used to assess whether it had ...
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Very Good Antibody for Detection of Plasmacytoid Dendritic Cells
(1)
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July 28, 2015
We are interested in the role of antigen presenting cells during the induction of oral tolerance in mice. For this purpose we analyze ...
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Excellent ADAM10 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 28, 2015
The aim of the present experiment was to test the time course effect of VEGFD on ADAM10 expression in human endothelial cells. We have ...
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Review for Rabbit Anti-MDA5 Antibody
(1)
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Thermo Fisher Scientific
July 28, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Great Antibody for Gamma Delta Cells
(1)
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July 27, 2015
We are interested in the contribution of gd T cells in the induction of tolerance. We choose most of our FACS antibodies from eBioscience ...
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Review for Rabbit Anti-RIG-I Antibody
(1)
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Cell Signaling Technology
July 27, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Rabbit mAb Anti-NUP98
(1)
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Cell Signaling Technology
July 27, 2015
This antibody was used to perform immunofluorescence microscopy on HeLa cells. A transfected FLAG-epitope tagged NUP62 was used a positive ...
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Small Scale Plasmid Isolation Kit
(1)
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July 27, 2015
We use this kit to isolate plasmid from 4 ml bacterial cultures. We generally use this kit to isolate good quality plasmid for validation ...
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