Time Course Effect of Chemotactic Agent on CCR4 in HASMS

August 18, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

R&D Systems

Product Name:

C-C chemokine receptor 4 (CCR4)

Catalog Number:

AF5207

Info: View Product View Product Specs

Browse Similar Products: CCR4 Antibodies

CCR4 is a cell surface receptor mediated by the G protein receptor family. It plays a significant role in angiogenesis, the immune system, nervous system, and homeostasis. Here we studied the time course effect of chemotactic agents on CCR4 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cell line

Primary Incubation

1:1000 in 5% skim Milk prepared in 1X TBST

Blocking Agent

5% skim Milk (blotting grade blocker) in 1X TBST

Secondary Incubation

1:4000 in 5% skim milk prepared in 1X TBST

Tertiary Incubation

NA

Detection

Bovine Anti-sheep

Results Summary

There is no change in the expression level by chemotactic agent.

Additional Notes

None

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Summary

The Good

This antibody is supplied with very high concentrations compared to other antibodies.

The Bad

It gives lot of background and the signal was very feeble.

The Bottom Line

This antibody gives a lot of background and non-specific bands and the signal is also very feeble even though it comes up with high concentration. I would not recommend this antibody for western blot.

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