Applied Biosystems
RNase Inhibitor
N8080119
The objective of the experiment was to synthesize cDNA from RNA which was isolated from liver tissue by TRIZOL method.
cDNA synthesis
Total RNA dissolved in nuclease free water
Total RNA obtained after the TRIZOL method was used for cDNA synthesis. To the master mix (containing nuclease-free H2O, buffer, primers, dNTP mix and reverse transcriptase), 1 μL of RNase Inhibitor was mixed along with the RNA sample. The contents were mixed properly and incubated in a thermocycler at 25°C for 10 min, 37°C for 120 min, 85°C for 5 min and 4°C for infinity. The reverse transcription product (cDNA) were used for downstream applications or stored at -20˚C.
None
Quality of results was good
Good quality cDNA was observed in PCR amplification
RNase inhibitor from applied biosystem showed the best RNA integrity and I recommend it; it must be used during cDNA synthesis.