The non-viral transfection of genetic material into cells, also known as gene delivery, is simplified by commercially available reagents and kits. The appropriate method for gene delivery depends on the type of cell and genetic material. Plasmid DNA, siRNA, proteins, and antibodies are transfected into cells using methods such as liposomes, calcium phosphate, and electroporation. Proficient stable or transient cell lines are generated using established cell lines, primary cells or stem cells with the appropriate transfection method. In vivo transfection reagents are offered with markers, such as Neomycin resistance. In addition to basic transfection kits, enhancement compounds can be used to increase the efficiency of the selected transfection treatment. A diverse range of transfection products is available to match the required gene delivery method.
Critical factors for choosing the right method for your research
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Tips for deciding whether or not to use hybridization capture
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Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR ...
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Hold your research and proof of concept studies to the highest standards with reliable and efficient electroporation technology. Features:
Achieve high efficiency and cell viability
Rapidly transfect from 75 thousand to 700 million cells
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The Neon Transfection System 100 µL Kit was used with the Neon Transfection System to deliver siRNA into primary immune cells, and the results have been excellent and highly reproducible.
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Lipofectamine has been my default choice whenever transfection efficiency is critical. It consistently delivers strong expression levels with straightforward protocols and minimal troubleshooting in commonly used cell lines. The main drawback is cost...
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