The non-viral transfection of genetic material into cells, also known as gene delivery, is simplified by commercially available reagents and kits. The appropriate method for gene delivery depends on the type of cell and genetic material. Plasmid DNA, siRNA, proteins, and antibodies are transfected into cells using methods such as liposomes, calcium phosphate, and electroporation. Proficient stable or transient cell lines are generated using established cell lines, primary cells or stem cells with the appropriate transfection method. In vivo transfection reagents are offered with markers, such as Neomycin resistance. In addition to basic transfection kits, enhancement compounds can be used to increase the efficiency of the selected transfection treatment. A diverse range of transfection products is available to match the required gene delivery method.
Pros and cons on each technique and tips on selection
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For most labs, the benefits outweigh any perceived drawbacks
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Our InvivO2 workstation is packed with innovative features that allow you to study even the most complex cell interactions under perfect physiological oxygen conditions. Whether you’re hoping to replicate the environment of blood vessels or lung ...
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Hold your research and proof of concept studies to the highest standards with reliable and efficient electroporation technology. Features:
Achieve high efficiency and cell viability
Rapidly transfect from 75 thousand to 700 million cells
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Lipofectamine has been my default choice whenever transfection efficiency is critical. It consistently delivers strong expression levels with straightforward protocols and minimal troubleshooting in commonly used cell lines. The main drawback is cost...
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I often transiently overexpress proteins in U2OS cells and found Lipofectamine 3000 to be the best transfection reagent for this cell line.
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