Quantitative ELISA - Murine Immunoglobulin Standard Coating Antibody

August 12, 2015

Vaccine & Virology Research
Harvard Medical School
Research Fellow

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Company:

AbD Serotec

Product Name:

Goat Anti-Mouse Kappa Light Chain

Catalog Number:

105001

To measure the concentrations of antigen-specific immunoglobulins by ELISA, an immunoglobulin standard curve is required. The coating antibodies for the capturing of murine immunoglobulins is an important consideration. This Goat Anti-Mouse Kappa Light Chain antibody provides accurate, consistent standard curves for murine quantitative ELISAs.

Experimental Design and Results Summary

Applications

ELISA

Sample

Purified immunoglobulin

Primary Incubation

Overnight at 4C. Diluted 1:3200 in 1x PBS. Coated at 100ul per well.

Blocking Agent

1x PBS, 1% BSA, 0.05% Tween-20

Secondary Incubation

Purified Ig diluted in 1x PBS, 1% BSA, 0.05% Tween-20 for 1hr at 37C

Tertiary Incubation

Goat anti-mouse Ig diluted in 1x PBS, 1% BSA, 0.05% Tween-20 for 1hr at 37C

Detection

Goat Anti-Mouse Immunoglobulin

Results Summary

The use of this Goat Anti-Mouse Kappa Light Chain antibody, in concert with AbD serotec's Goat Anti-Mouse Lambda Light Chain, as the coating antibodies provide the basis for a murine immunoglobulin standard. Used with the provided conditions, standards with R2-values of above 0.998 are consistent.

Additional Notes

None

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Summary

The Good

Availability, price, efficacy

The Bad

None

The Bottom Line

A good coating antibody when used in combination with Goat Anti-Mouse Lambda Light Chain (AbD serotec cat: 106001) for a murine immunoglobulin standard required for antigen-specific murine quantitative ELISAs.

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