Wako Pure chemicals
Bambanker Freeze medium
NC9582225
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This product is intended to preserve viable cells for long-term storage under freezing conditions (-80C or liquid N2). It is a better alternative to 90%serum with 10%DMSO (traditional freeze medium), as it's free of toxic chemicals and I have noticed better viability even with primary cells.
Freezing cells
Cells
Pellet the cells of interest and remove all supernatant. Resuspend the pellet in Bambanker media and transfer to cryo-vials for freezing in -80 freezer or liquid nitrogen.
Use slow cooling chambers for better viability
I have noticed up to 80% viability in cells after thawing compared to 50-60% viability with traditional freezing media.
None
Easy to use and convinient
Expensive alternative
If you have precious cells or cell lines, this is a good medium for preserving them.
Product Name: Bambanker from Wako Chemicals USA, Inc.Supplier Page