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Date Published
THE™ V5 Antibody
(1)
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July 31, 2015
This antibody was used to perform immunofluorescence microscopy on SW480 cells transfected with a V5-containing construct. This antibody was...
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Facilitated RNA extraction
(1)
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QIAGEN - Sample to Insight
July 31, 2015
Extracting RNA from cell lines or from tissue specimens has the historic connotation of being an arduous process. This kit, from Qiagen, ...
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Beware... if you're shopping for Autoclaves!
(1)
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July 30, 2015
The Getinge Model 522LS Sterilizer/Autoclave is a cost-competitive instrument that appealed to my department. Unfortunately, in the 5 years...
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Ki-67 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
July 30, 2015
The Ki67 antibody was used for IHC in the prostate to validate that the cancer cells are actively proliferating. Ki67, when this occurs, is...
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Immunocytochemistry of a Her2 Variant
(1)
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R&D Systems, a Bio-Techne Brand
July 30, 2015
We are studying Her2 expression in a number of cancer cell lines that may express Her2 variants. We selected this antibody because it ...
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Review for Mouse Anti-ß-Actin Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 30, 2015
Protein extract from MEFs was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Review for Rabbit Anti–Phospho-IRF3 Antibody
(1)
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Cell Signaling Technology
July 30, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Ninja TAQ Found be a Very Robust and Sensitive Enzyme for Amplification
(1)
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Pharmozyme
July 29, 2015
This enzyme was found to be better than any other commercially available TAQ polymerase. It works well for 200bp product amplification. Also...
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Detection of PGP in Colon Cancer Cells
(1)
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Abcam
July 29, 2015
One of the known methods of developing tumor resistance is via the expression of PGP. Here we test if the antibody can detect PGP in colon ...
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Good Kit for Cleaning the Reaction Components
(1)
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QIAGEN - Sample to Insight
July 29, 2015
We use this kit for cloning purpose. The amplified PCR product is purified from total reaction mixture. The purified PCR product is directly...
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Review for Rabbit Anti-IRF3 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 29, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Real Time PCR with High Efficiency
(1)
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Analytik Jena
July 29, 2015
We are interested in minimum residual disease also called MRD. We used the qTOWER for our studies on MRD and we gained 2 points for Ct ...
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Pierce ECL Plus Western Blotting Substrate
(1)
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July 28, 2015
This product was used to check the protein (transcription factors pCREB etc) expression changes in neuronal activity. This product generates...
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Pharmacologically Active In Vivo Use of Romidepsin
(1)
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Selleck Chemicals
July 28, 2015
To use HDAC inhibitors in an attempt to recapitulate HIV-1 latency reversion, but in mice. Romidepesin was used to assess whether it had ...
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Very Good Antibody for Detection of Plasmacytoid Dendritic Cells
(1)
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July 28, 2015
We are interested in the role of antigen presenting cells during the induction of oral tolerance in mice. For this purpose we analyze ...
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Excellent ADAM10 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 28, 2015
The aim of the present experiment was to test the time course effect of VEGFD on ADAM10 expression in human endothelial cells. We have ...
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Review for Rabbit Anti-MDA5 Antibody
(1)
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Thermo Fisher Scientific
July 28, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Great Antibody for Gamma Delta Cells
(1)
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July 27, 2015
We are interested in the contribution of gd T cells in the induction of tolerance. We choose most of our FACS antibodies from eBioscience ...
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Review for Rabbit Anti-RIG-I Antibody
(1)
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Cell Signaling Technology
July 27, 2015
Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with ...
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Rabbit mAb Anti-NUP98
(1)
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Cell Signaling Technology
July 27, 2015
This antibody was used to perform immunofluorescence microscopy on HeLa cells. A transfected FLAG-epitope tagged NUP62 was used a positive ...
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Small Scale Plasmid Isolation Kit
(1)
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July 27, 2015
We use this kit to isolate plasmid from 4 ml bacterial cultures. We generally use this kit to isolate good quality plasmid for validation ...
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Good Quality DNA for Cloning
(1)
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QIAGEN - Sample to Insight
July 27, 2015
We use this kit to purify the digested Plasmid DNA have the gene of interest during the cloning procedure. we use this kit for maximum ...
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Review for Anti-PKD1/PKCµ Antibody
(1)
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Cell Signaling Technology
July 24, 2015
Cells were washed twice in PBS and harvested with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-...
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Time Course Effect of PAR-3 in Macrophages
(1)
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Santa Cruz Biotechnology, Inc.
July 24, 2015
PARs are the thrombin receptors that are activated by the G proteins. In this study we have to study the effect of thrombin on PAR-3 ...
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pERK Expression in Neuronal Cells (N2A)
(1)
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Abcam
July 24, 2015
This product was used to check the expression of pERK in Neurotrophicy of N2A cells.
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TrkB Inhibitor to Check BDNF-TrkB Induced Neurotrophicity
(1)
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July 24, 2015
We used this product to check the role of BDNF-TrkB in neurotrophic activity. The product is specific to the TrkB inhibition.
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My Favourite Sequencing Service
(1)
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July 24, 2015
Genewiz has wonderful customer service, excellent sequencing results, even with difficult templates, and accurate readouts. Competitive ...
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Very Good Substrate for Developing Blots
(1)
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July 24, 2015
We use this kit to develop blots using storm 860 phosphoimager as the imaging system. ECL Plus reagent has high sensitivity and a longer ...
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Multi-Color Protein Standard
(1)
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Bio-Rad
July 23, 2015
I use this product when running protein gels. I like this product because it is already mixed so I just have to add the solution to the gel ...
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Anti-Mouse/Rat Foxp3 Foxp3-APC
(1)
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July 23, 2015
Anti-Mouse/Rat Foxp3 Foxp3-APC was used to stain blood lymphocytes from rats with developed cancer. Percentage of Foxp3 positive cells was ...
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Excellent Antibody for Staining of Dendritic Cells
(1)
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July 23, 2015
Our lab is interested in the role of antigen presenting cells during oral tolerance induction. We analyze the phenotype and function of ...
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Aquaporin 2 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 23, 2015
My project involves understanding the physiological roles of Eps15 Homology domain-containing protein 4 (EHD4) in the kidney. EHD4 is an ...
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Excellent Antibody to Detect SirT1 in Human Cell Lines
(1)
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Cell Signaling Technology
July 23, 2015
The aim of the study was to test the time course effect of VEGFA on SirT1 expression in human endothelial cells. We found Cell Signaling ...
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YLPM1 antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
July 22, 2015
The antibody works perfectly fine and shows a band of the desired molecular weight.
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B220_B-cell stain
(1)
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BD Biosciences
July 22, 2015
I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various ...
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Excellent Antibody for pSTAT3 (Tyr705)
(1)
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Cell Signaling Technology
July 22, 2015
The aim of the experiment was to test the effect of dominant, negative (tyr705) STAT3 (Ad-dnSTAT3) on STAT3 (Tyr705) phosphorylation in ...
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Review for Anti-TNFR1 antibody (ab19139)
(1)
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Abcam
July 22, 2015
Hepatocytes were washed with cold phosphate-buffered saline (PBS), collected in lysis buffer (Cell Signaling Technology), sonicated, and ...
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Alcohol Dehydrogenase (for Western Blot) from Novus Biologicals, Inc.
(1)
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Novus Biologicals, a Bio-Techne Brand
July 22, 2015
This antibody was used in a study that investigated the role of acetaldehyde in alcohol induced lipodystrophy in rats. It has been published...
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Good Transfection Reagent
(1)
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Thermo Fisher Scientific
July 21, 2015
Aim of the experiment was to deplete NFATc1 at protein level in endothelial cells. For this, NFATc1 siRNA at different molal concentration ...
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Good Quality Real-Time PCR System
(1)
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July 21, 2015
We have 7500 fast Real time PCR system in our central lab equipment facility. Aim of my experiment was to study the quantitative expression ...
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Review for Anti-GAPDH antibody - Loading Control
(1)
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Abcam
July 21, 2015
Hepatocytes were washed with cold phosphate-buffered saline (PBS), collected in lysis buffer (Cell Signaling Technology), sonicated, and ...
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VECTASHIELD Antifade Mounting Medium with DAPI
(1)
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July 21, 2015
My project involves understanding the physiological role of Eps15 Homology domain containing protein 4 (EHD4) in the kidney. EHD4 is ...
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FMN2 antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
July 21, 2015
The antibody gives a single major band ~80 kDa. It is difficult to predict and conclude if it the correct FMN2 protein with reduced ...
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Review for Anti-TACE antibody (ab39163)
(1)
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Abcam
July 20, 2015
Snap-frozen liver (median lobe) was homogenized in lysis buffer and centrifuged at 16,000g for 15 min, after which the supernatant was ...
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Oil Red O Stain Kit
(1)
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American Master* Tech Scientific, Inc.
July 20, 2015
We used this product to check liver lipogenesis condition.
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Isolation of Naive CD4+ T Cells, Mouse
(1)
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Miltenyi Biotec
July 20, 2015
This kit is used for isolating naive CD4+ T cells from mice in a two step MACSing procedure, based on the expression of CD4+ and CD62L+. The...
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Best Antibody to Detect Ac-STAT3 (Lys685) in Human Cells
(1)
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Cell Signaling Technology
July 20, 2015
The aim of our research was to test the time course effect of VEGFB on STAT3 (Lys685) acetylation in human endothelial cells by ...
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Picro Sirius Red Staining Kit
(1)
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July 20, 2015
We used this product for collagen histochemistry.
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Great Anti-Laminin Antibody
(1)
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Abcam
July 17, 2015
We wanted to localize/visualize the basement membrane. As laminin is a protein found in the basement membrane we used this ab to visualize ...
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Review of Anti-XIAP antibody
(1)
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July 17, 2015
To perform Western Blot analysis, cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1...
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Western Blot
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Cell Fractionation
Immunoassay
In Vitro Transcription
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3D Imaging
RNAi
ELISPOT
PCR Cloning
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Protein Modification
DNA Analysis
Expression
Cell-Based Assay
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In Vivo Testing
Protein Labeling
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Human
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Adipose
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Cell Type
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Adipocyte
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Microvascular Endothelial Cell
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Platelets
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Tumor Cells
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HeLa
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A431
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N2a
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