Time Course Effect of Chemotactic Agent on Phospho-PLC-gamma-1 in HASMS

August 21, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling

Product Name:

Phospho-PLCγ1

Catalog Number:

2821

Phosphoinositide-specific phospholipase C (PLC) is a receptor of the tyrosine kinase family which is activated upon various stimuli like growth factors and hormones. Phosphorylation at Tyr783 is one of the characteristic features of the PLC. Once it gets activated and further catalyzes and hydrolysis the phosphatidylinositol 4,5-biphosphate to inositol 1,4,5-triphosphate and diacylglycerol it plays an important role in cell proliferation, migration and differentiation. Here we checked the effect of Phospho-PLCγ1 by a chemotactic agent.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cell line

Primary Incubation

1:500 in 5% BSA prepared in 1X TBST

Blocking Agent

5% BSA from Sigma prepared in 1X TBST

Secondary Incubation

1:4000 in 5% skim milk prepared in 1X TBST

Tertiary Incubation

NA

Detection

GAR

Results Summary

There is no change in the expression level by the chemotactic agent.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

There is no/less background, the antibody is very efficient, and the signal is very good.

The Bad

Its gives some non-specific bands.

The Bottom Line

This antibody is very efficient and the signal is very good. It is highly recommended for performing western blot.

Share your experience with other scientists. Write a Review! »

Join the discussion