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Date Published
Easy kit for plasmid midipreps
(1)
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Omega Bio-Tek, Inc.
June 18, 2015
I perform a lot of cloning in the lab and use this kit to isolate plasmid DNA from bacteria. I like using this kit because it is pretty easy...
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Fast and easy but do not let the warranty expire
(1)
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Bio-Rad
June 18, 2015
The trans-blot turbo system is easy and fast to use especially if combined with the Bio-rad transfer kit that combines all the solution, ...
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Anti-Canine CD25 PE
(1)
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June 18, 2015
CD25 was used as a marker to identify CD4+FoxP3+ regulatory T cells in dogs with cancer. A significant increased number of Tregs was found ...
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Anti-6X His tag antibody
(1)
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Abcam
June 18, 2015
MCF7 cells were grown in DMEM medium supplemented with 10% FBS, 100µg/ml streptomycin and 100µg/ml penicillin in 5% CO2 at 37°C. Cells were ...
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Easy-to-use Retroviral Titer kit
(1)
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June 18, 2015
Retroviral quantification is critical especially when combined with other biomolecular assays for precise control of signaling mechanisms. ...
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Normicn in mammalian cell culture media to prevent microbial contamination
(1)
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June 17, 2015
We are performing compound toxicity and signalling studies on diverse neuronal cell lines. Some cells like neuro2A are highly sensitive to ...
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The best LAP antibody for humans that I have found
(1)
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BioLegend
June 17, 2015
This antibody detects membrane bound latent TGF-b1 by staining of the latency associated peptide (LAP). I use only 5ul instead of the ...
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Efficient Reverse Transcription via Taqman Reagents leads to precise quantification of retroviral titer
(1)
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June 17, 2015
Quantification of retroviral titer can be tricky considering that they contain RNA as the genetic construct rather than DNA. Conversion of ...
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High Fidelity Polymerase Product
(1)
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June 17, 2015
I chose this product because PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase for PCR amplification, it can be used for GC-rich ...
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Stable and efficient reducing of liposomal size
(1)
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Avanti Polar Lipids Inc.
June 17, 2015
Development of liposomal formulations for gene therapy and drug delivery is a common technique used in most transfection protocols. In order...
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Convenient but not made very well
(1)
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June 16, 2015
As with any lab, we do quite a bit of centrifuging. We purchased this Mini Centrifuge because of its convenience and its ability to fit a ...
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Good anti-mouse CD11b antibody
(1)
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Thermo Fisher Scientific
June 16, 2015
The objective of this experiment was to identify the percentage of CD11b positive cells in the splenocytes isolated from Balb/C mouse. To ...
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pCREB expression in N2A cells
(1)
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Abcam
June 16, 2015
Our work focuses on gene and protein changes (epigenetic modulators) involved in neurotrophic and neuroprotective activity. We used this ...
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A good antibody for platelet identification
(1)
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June 16, 2015
This antibody is generally used to identify platelets in the whole blood. In combination with the forward scatter/side scatter, this ...
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Precise Viral RNA Isolation from supernatant of retroviral producer cell lines
(1)
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QIAGEN - Sample to Insight
June 16, 2015
Producer cell lines to obtain retroviruses and adenoviruses are commonly used in most viral therapeutics and transduction labs. In order to ...
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Very good anti-Mouse CD8 antibody
(1)
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June 15, 2015
The aim of this experiment was to study the T cell subsets in mouse spleen. I tested whether this anti-CD8 PE/Cy7 antibody from BD ...
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pCREB expression in Neuronal cells
(1)
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MilliporeSigma
June 15, 2015
We used this product to check the pCREB expression in neuronal cells and its role in neural functioning. Compared to other company's ...
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Brilliant fluorochrome for a dim marker
(1)
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BioLegend
June 15, 2015
This antibody was used in a staining panel to identify Tregs in the thymus and the spleen. The CD25-Brilliant Violet 421 antibody had a ...
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Precise elemental composition in a timely fashion provided by Atlantic Microlab, Inc
(1)
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June 15, 2015
Our lab works with formulation of various drug salts for testing therapeutic efficiencies and pharmacology. Apart from the host of other ...
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Expression of activation marker CD69 by flow cytometry
(1)
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BioLegend
June 15, 2015
T cells and NK cells upregulate surface marker CD69 upon activation. CD69 is one of the early activation markers in T cell stimulation/ ...
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Nkp46 IFC detection
(1)
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R&D Systems, a Bio-Techne Brand
June 12, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. In this ...
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Lympholyte M
(1)
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CEDARLANE
June 12, 2015
We use lympholyte M as centrifugation step in preparation of bone marrow derived dendritic cells.
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Anti-Human IgG1 antibody
(1)
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GeneTex
June 12, 2015
I used this antibody to screen my protein of interest in culture supernatants which had an IgG1 Fc tag. Earlier, with other anti IgG ...
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Isolation of RNA from T cells
(1)
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June 12, 2015
The purpose for using this kit was to isolate RNA from a small number of T cells. I chose it because it seemed easy to use, quick, and it ...
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Transfection quality plasmid purification kit
(1)
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QIAGEN - Sample to Insight
June 12, 2015
We used this kit to purify our plasmid of interest to transfect into mammalian cell lines. This kit provides a high quality plasmid with ...
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Good anti-Mouse CD4 antibody
(1)
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BioLegend
June 11, 2015
The objective of this experiment was to study T cell subsets in mouse spleens. I tested if the anti-CD4 APC/Cy7 antibody from BioLegend can ...
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OCT4 antibody for iPS cells
(1)
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June 11, 2015
I work with human iPS cells and use several antibodies for pluripotency verification. One of them is the OCT4 antibody from Stemgent. I ...
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Vector for TA cloning
(1)
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Promega
June 11, 2015
We use this kit for cloning genes. By using this kit, we can select specific pairs of restriction enzyme sites at both the 5' and 3' ends of...
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Good anti-Mouse CD45 antibody
(1)
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BioLegend
June 11, 2015
We used this antibody to determine CD45 expression in mouse splenocytes. Single cell suspensions isolated from mouse splenocytes were used ...
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CyclinB1 is a very good antibody for Western blot with out any non specificicity
(1)
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June 11, 2015
This product was used in a study to screen anticancer drugs that can inhibit G2/M phase of the cell cycle, in this case CyclinB1 is used as ...
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Good quality DNA for cloning
(1)
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Illumina
June 10, 2015
We purify DNA from mouse blood for cloning purposes. We tend to get lot of RNA contamination by conventional phenol-chloroform methods. We ...
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If you do a lot of ELISA work, this will be a good companion
(1)
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Agilent Technologies
June 10, 2015
We do a lot of ELISA in our lab. One very tedious part of ELISA is to wash the plate. Not only it takes a lot time, it also wastes a lot of ...
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Menin Antibody for Western Blotting
(1)
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Bethyl Laboratories, Inc.
June 10, 2015
We were interested in looking at the protein levels of menin as well as its interacting partners in prostate cancer cell lines (VCaP and ...
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Co-formulation of a hybrid retroviral gene therapy vector using a fluorescent lipid for applications in microscopy
(1)
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Avanti Polar Lipids Inc.
June 10, 2015
Visualization of gene therapy vectors is critical for quantitative evaluation of their functional performance in various cells. Here, we ...
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This gel extraction kit has high quality with low price
(1)
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June 09, 2015
This kit contains 250 preparations, the price is low, but the quality is good enough for PCR product recycling and digestion product ...
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Recovery of Retroviral Transduction via DOTAP
(1)
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Avanti Polar Lipids Inc.
June 09, 2015
Retroviruses are commonly used gene therapy vectors but can be extremely unstable, inefficient and expensive due to sensitivities in ...
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Accurate quantification of RNA concentration
(1)
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Thermo Fisher Scientific
June 09, 2015
I use this kit for measuring RNA concentration before cDNA synthesis with reverse transcription. Obtaining accurate RNA concentration is ...
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Magnetic Isolation of Regulatory T cells using RosetteSep
(1)
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June 09, 2015
The goal of my research is to determine changes in regulatory T cell populations within the peripheral blood following treatment. I chose to...
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Alpha tubulin expression in HELA cells
(1)
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June 09, 2015
This product was used as part of a study to check protein expression changes in cancer. The product worked well as a loading control.
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Plasma Membrane staining to quantify intracellular biochemistry via fluorescence
(1)
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Thermo Fisher Scientific
June 08, 2015
Plasma membrane staining is another way to counterstain for cellular assays apart from nuclear stains. FM 1-43 dye is a highly specific ...
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A must for RNA extraction
(1)
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June 08, 2015
I used this product to extract RNA from peripheral blood mononuclear cells. Since the sample collection took quite a while, I saved my ...
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To measure the HDAC activity/Inhibition
(1)
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EpiGentek
June 08, 2015
This product was used to measure HDAC activity/inhibition in samples treated with HDAC inhibitor.
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Western Blot with phospho-Tuberin S1387 antibody
(1)
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Cell Signaling Technology
June 08, 2015
This antibody was used to detect levels of phospho-tuberin S1387 in cancer cell lines. When loading 50 ug of cell lysate, we were able to ...
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DNA isolation using DNeasy kit
(1)
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QIAGEN - Sample to Insight
June 08, 2015
The aim of my research was to understand methylation status on genomic DNA isolated from CD4+ T helper cells. I wanted to use the DNeasy kit...
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Good anti-Mouse CD45 PE antibody
(1)
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June 05, 2015
The objective of this study was to standardize CD45 staining on mouse splenocytes. For this purpose single cell suspensions were isolated ...
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Transcriptomics for T cell differentiation
(1)
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June 05, 2015
The aim of my research was to isolate T cells from drug-treated mice and assess genomic changes within the T cell population following ...
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Easy to use for RNA cleanup
(1)
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QIAGEN - Sample to Insight
June 05, 2015
I am interested at the expression level of several inflammatory genes in peripheral blood mononuclear cell. I used this kit for RNA cleanup ...
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Efficient Nuclear Labeling of Live Cells
(1)
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Thermo Fisher Scientific
June 05, 2015
Primary tissues and cells are routinely analyzed for various biochemical and pharmacological behavior. One of the primary methods to ...
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HDAC2 expression in N2A cells
(1)
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Abcam
June 05, 2015
We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. This product was used ...
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Tonbo CD11c Red Fluor 710 antibody
(1)
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June 04, 2015
I used Tonbo CD11c for assessment of bone marrow-derived dendritic cells via fluorescence cytometry.
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