Bio-Rad
Clarity Western ECL Blotting Substrate
170-5060
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We have had difficulties getting a good signal on our Licor digital Western blot imager using standard ECL substrates. Bio-Rad's Clarity reagent solved this problem with a good signal after a shorter imaging time.
Western Blotting
PVDF membranes with cytoplasmic lysates
750 ul each of Clarity reagents A and B were mixed in an eppendorf tube and applied to a PVDF membrane containing cytoplasmic lysates (20ug loaded/lane). The lysates were from human B cell lines that were either untreated or stimulated overnight with monosodium ureate crystals. After electrophoresis in 4-12% of a Bio-Rad TGX gel, lysates were transferred via standard protocols. Membranes were probed with rabbit anti-human IL-6 polyclonal antisera, followed by a goat anti-rabbit secondary-HRP. Blots were imaged using a Licor C-Digit blot scanner for six minutes.
Our Gel-Doc imager can capture excellent blot images in < 10 seconds
Images from the experiment were clear with crisp bands and no background. The reagent solved a problem that had been plaguing several labs using standard ECL HRP substrates.
None
Clarity substrate gives signal detectable using digital imagers
None; Clarity is even less expensive than our previous reagent.
Excellent product and when combined with digital imaging, yields rapid results.
Product Name: Clarity™ Western ECL Substrate, 200 mlSupplier Page