Purify Untouched CD8 T Cells Efficiently

August 18, 2015

Brigham and Women's Hospital
Pathology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Stem Cell Technologies

Product Name:

EasySep Mouse CD8+ T Cell Isolation Kit

Catalog Number:

19853A

This product is for purifying mouse CD8 T cells from cell suspensions using magnetic negative selection, i.e. the magnetic beads bind to everything except CD8 T cells, meaning your population is actually untouched. It's a great kit for downstream processes that require pure populations without antibody staining.

Experimental Design and Results Summary

Application

Purification of CD8 T cells

Starting Material

Cell Suspensions

Protocol Overview

Follow the kit protocol and separate the populations using the magnetic separator.

Tips

For higher purity, extend incubation times

Results Summary

Highly pure population (>90% CD8+) was obtained after the purification as measured by Flow cytometry.

Additional Notes

None

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Summary

The Good

Fast and inexpensive

The Bad

Purity can vary from sample to sample

The Bottom Line

This is a high yield, reasonably priced kit for downstream processing. One could even use it for pre-purification to reduce sample volume followed by more stringent methods.

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