Novus Biologicals
WBP11
NBP2-20886
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Browse Similar Products: WBP11 Antibodies
Our current project is all about identification of affected proteins and their levels upon specific treatment of mammalian (HeLa) cells.
Western Blot
Untreated and treated Hela cells lysate 25 ug protein
1:500 overnight at 4 degrees Celsius
Blocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT
1:20,000 HRP-conjugated secondary, 60 minutes at room temp
NA
Femto Chemiluminescent
Major band comes ~95 kDa
None
The good thing is that at least you see your desired band
The bad part is you should not get confused with any faint band
If you have any other alternatives to this antibody, I would suggest you try that one first.