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Date Published
Nice ECL Reagent
(1)
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September 28, 2015
I am a molecular biologist and western blotting is a part of my routine. I have used this product since starting my research and it has ...
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Decent Viability Dye
(1)
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Thermo Fisher Scientific
September 28, 2015
This dye was used to discriminate between live and dead cells in experiments that needed fixation and permeabilization for intracellular ...
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Anti-Foxp3 Clearly Demarcates the Treg Population
(1)
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September 25, 2015
For this study, we wanted to examine fluctuations in the Treg proportions in the small intestine of mice in the healthy vs disease state. ...
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Good Phospo-NF-kb p65 Antibody for Western Blot and Flow Cytometry
(1)
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Cell Signaling Technology
September 25, 2015
I use this antibody to measure NF-kB activation in mouse CD4+ T cells after treatment with our stimuli. I was looking for an antibody which ...
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Good Antibody for This Dim Fluorochrome
(1)
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September 25, 2015
The APC-eFluor780/APC-Cy7 fluorochrome is known to be a pretty dim color so when choosing an antibody for this channel, we aim to find a ...
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cDNA Synthesis from mRNA Using Verso cDNA Synthesis Kit
(1)
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Thermo Fisher Scientific
September 25, 2015
This product was used to check the gene expression changes in glioblastoma cells in differential treatment conditions. The product is easy ...
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Kit for Measuring Glycosylated Hemoglobin (HbA1c) in Blood
(1)
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September 25, 2015
HbA1c was measured in blood samples of diabetic and non-diabetic rats by using this kit.
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Excellent Cleaning Agent for Removing RNase
(1)
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September 25, 2015
During RNA isolation, RNaseZAP is sprayed in the working area to avoid air contamination; surfaces and gloves were cleaned by wiping with ...
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Must Have Instrument for Imaging Blots, Gels, Cells, etc.
(1)
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LICORbio
September 25, 2015
We use western blots and in-cell westerns to analyze changes in protein expression or modification in tissues and cells. We have almost ...
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Good Western Blot Antibody for Human/Mouse/Rat STAT5
(1)
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Cell Signaling Technology
September 25, 2015
I used this antibody to measure STAT5 activation in mouse CD4+ T cells upon treatment with our stimuli. I was looking for an antibody which ...
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Good Western Blot Antibody
(1)
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September 24, 2015
I use this antibody to measure NF-kB activation in mouse CD4+ T cells upon treatment with our stimuli. This antibody was comparatively ...
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Simple to Use Non-Hazardous G-250 Coomassie Stain
(1)
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Thermo Fisher Scientific
September 24, 2015
After electrophoretic separation of protein samples, it is often useful to stain the gel for proteins. While many companies offer non-...
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Excellent Antibody for Detecting HA Epitope Tag
(1)
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BioLegend
September 24, 2015
This antibody detects HA epitope tagged proteins with great ease in a variety of human and mouse cell types.
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Good PVDF
(1)
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September 24, 2015
This product is used in western blot applications. Specifically, proteins are transferred from polyacrylamide gels to this PVDF and then ...
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Excellent Antibody For Staining of FoxP3+ Regulatory T Cells
(1)
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September 24, 2015
Our laboratory is interested in induction of tolerance in autoimmune diseases. Tolerance induction is often associated with induction of ...
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Western Blot Antibody for p50
(1)
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Cell Signaling Technology
September 24, 2015
I used this antibody to measure NF-kB activation in mouse CD4+ T cells upon stimulation with our treatment. I was looking for an antibody ...
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DMEM/F12 Media
(1)
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Thermo Fisher Scientific
September 24, 2015
To culture the glioblastoma stem cells. The media is ready-made, saves the time and less chances of contamination.
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Can Perform Every Liquid Handling Operation You Can Imagine
(1)
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Thermo Fisher Scientific
September 23, 2015
Like most laboratories, we predominantly assay in 96-well format so a multichannel pipette is indispensable. One advantage of this ...
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Culture Media for Growing Airway Epithelium at Air-Liquid Interface
(1)
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Lonza
September 23, 2015
We use this media to grow bronchial and nasal epithelium at air-liquid interface conditions. The media is very easy to make, you just add ...
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Great Antibody for CD25, the Low-Affinity IL-2 Receptor alpha-Chain
(1)
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BD Biosciences
September 23, 2015
Our laboratory is interested in peripheral tolerance. CD25 (low-affinity IL-2 Receptor a chain) is expressed on activated T and B ...
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Good Alternate Fluorochrome for the AmCyan Channel
(1)
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BD Biosciences
September 23, 2015
I tried the anti-TCRbeta (clone H57) antibody conjugated to Brilliant Violet 510 when designing a multicolor panel to identify specific T ...
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Multistir Platform
(1)
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Bellco Biotechnology
September 23, 2015
This is a good platform for mixing solutions because you can mix 4 at once. This can save time if you need to make multiple solutions.
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X-Gal for Bacteriological Assays
(1)
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September 23, 2015
I screened a lung cDNA library for a potential ESAT6 binder using bacterial two hybrid system. We used X-gal selection plate to screen for ...
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Bad Immunodetection of FGF2
(1)
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Santa Cruz Biotechnology, Inc.
September 23, 2015
Proteins were extracted from mammalian cell lines treated with growth factor. Equal amount of proteins were resolved in 12% SDS-PAGE and ...
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Good HRP Conjugated Beta-Actin Antibody
(1)
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Santa Cruz Biotechnology, Inc.
September 23, 2015
I use this antibody as an internal control for my quantitative blots. Initially, I was using a beta-actin antibody from another supplier ...
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PCR Master Mix With Loading Dye
(1)
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Genesee Scientific
September 22, 2015
I have used Taq Master Mix from Apex (sold by Genesee Scientific) for several years and have always had excellent results. It essentially ...
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DEPC Water
(1)
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September 22, 2015
This water is used for nuclease free applications such as eluting RNA from a purification column. After using this product several times I ...
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Phosphatase Inhibitor
(1)
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Roche
September 22, 2015
This cocktail is used to inhibit phosphatases in protein lysates being assessed for phospho-proteins by western blotting. Easy to dissolve ...
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Western Blot Analysis of FASN
(1)
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Santa Cruz Biotechnology, Inc.
September 22, 2015
Our laboratory is focused on studying the role of EGFR in de novo fatty acid synthesis and mitochondrial dynamics. This product was used to ...
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TCRb PE FACS Antibody
(1)
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BD Biosciences
September 22, 2015
I use this antibody to gate specifically on T cells in order to distinguish between gd T cells and ab T cells in the gut. In addition, I use...
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Extremely Strong Antibody Against Biotin
(1)
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Abcam
September 22, 2015
This antibody detects trace amounts of biotinylated proteins with great ease at the recommended dilution.
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Rhot1 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
September 21, 2015
We had previously used other Rhot1 antibodies to determine the levels of Rhot1 in human and mouse lungs. We were not able to get clear bands...
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Excellent Antibody for Detecting Endogenous Ezh2
(1)
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Cell Signaling Technology
September 21, 2015
This Cell Signaling Rabbit Ezh2 antibody detects small amounts of endogenous Ezh2 with great ease at the recommended (1:1000) dilution.
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Protease Inhibitor Cocktail
(1)
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Roche
September 21, 2015
Use this protease inhibitor cocktail when making protein lysates for western blotting. Easy to dissolve tablets make it easy to make the ...
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Sensitive But Time-Consuming Protein Assay
(1)
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September 21, 2015
Measuring protein concentration is one of the first steps in our workflow. We use this assay because its 96 well microplate format works ...
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gd TCR PerCP-eFluor 710 for FACS
(1)
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September 21, 2015
I preferentially use this antibody to define gd T cells in the colon. For this, I firstly isolate the cells of the colon with a common cell ...
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Good Mounting Media
(1)
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Electron Microscopy Sciences
September 21, 2015
This product is used to mount fluorescent specimens onto slides to reduce signal loss. It is a ready to use product with no dilutions or ...
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Nice Kit to Purify PCR Products
(1)
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QIAGEN - Sample to Insight
September 18, 2015
This kit is used to purify PCR products after performing PCR. The kit removes primers as well as proteins and samples run through the kit ...
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Western Blot Analysis of Na+/K+-ATPase Alpha 1
(1)
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Santa Cruz Biotechnology, Inc.
September 18, 2015
We used this product as a plasma membrane marker protein for our subcellular fractionation assays.
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Works Poorly in Mouse Cells
(1)
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Abcam
September 18, 2015
I tried this antibody in Western Blot studies using mouse embryonic fibroblast cells and observed that it poorly detects Dnmt1 protein at ...
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Great Secondary Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
September 18, 2015
We study sinus disease in humans, and specifically we were looking at epithelial tight junction staining in ex vivo cultured epithelium.
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Anti-Gamma H2A.X Antibody
(1)
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Abcam
September 18, 2015
We are using human dental fibroblast cells study oxidants. We measure oxidant induced DNA damage by induction of H2AX in cell lysates.
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HDAC2 Antibody for Immunoblotting
(1)
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Abcam
September 17, 2015
We tested different HDAC2 human antibodies for our samples and we found ab32117 antibody works good for human tissue lysate and cell lysates...
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Good Native Sample Buffer
(1)
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Bio-Rad
September 17, 2015
I use this buffer when running a native sample gel i.e. non-denaturing. Sample buffer comes in 2X stock that you just dilute 1:1 with sample...
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Mitotracker Staining Before Fixation
(1)
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Thermo Fisher Scientific
September 17, 2015
The cell-permeant MitoTracker® contain a mildly thiol-reactive chloromethyl moiety and is used in live cells for labeling mitochondria. ...
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Good Kit for Silver Staining
(1)
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Thermo Fisher Scientific
September 17, 2015
This is a good easy to use kit for silver staining protein samples. I have used this kit to detect femtomol concentrations of proteins and ...
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Excellent Antibody to Detect Pak2 in Mammalian Cell Line
(1)
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Cell Signaling Technology
September 17, 2015
Pak2 levels were detected in mammalian cells treated with growth factor. Equal proteins were resolved, western transferred and probed for ...
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Reliable primary antibody in western blot analysis
(1)
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Cell Signaling Technology
September 17, 2015
We carry out subcellular fractionation and verify the isolated fractions by western blot analysis. We have had good experience with Cell ...
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Excellent Intracellular Staining Antibody
(1)
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R&D Systems, a Bio-Techne Brand
September 16, 2015
We were interested in looking at the expression of DAP-12 protein in specific subset of myeloid cells from human PBMCs. DAP-12 monoclonal is...
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Anti-HDAC2 (Phospho S394) Antibody for Mouse
(1)
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Abcam
September 16, 2015
Anti-HDAC2 phospho S394 antibody for mouse lung tissues. Mouse exposed to cigarette smoke shows increased HDAC2 phosphorylation compared to ...
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