Time Course Effect of Thrombin Induced NPC1 Expression in Macrophages

August 11, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Niemann-Pick disease, type C1 (NPC1)

Catalog Number:

sc-18201

Info: View Product View Product Specs

Browse Similar Products: NPC1 Antibodies

Niemann-Pick disease, type C1 is caused by mutations in the NPC1 gene. It is also called a lipid storage disorder caused by altering the lipid metabolism and accumulation of lipids. Here we studied the time course effect of thrombin induced NPC1 expression in macrophages.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Cell lysate

Primary Incubation

1:500 in 5% Milk prepared in 1X TBST

Blocking Agent

5% Milk(blotting garde blocker) in 1X TBST

Secondary Incubation

1:4000 in 5% Milk prepared in 1X TBST

Tertiary Incubation

NA

Detection

Bovine Anti-Goat

Results Summary

Our results showed that there is no change in the expression level by thrombin.

Additional Notes

None

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Summary

The Good

There is no/less background, the antibody is very efficient and the signal is very good.

The Bad

Some non-specific bands detected with high intensity and to get the signal, it takes some time.

The Bottom Line

The antibody is very good and the signal is strong enough. It takes some time to to get the signal and we have to run at least 8% gel for nice separation to get the appropriate band. Overall, it is a highly recommended antibody for western blot.

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