CD4 Stain BV711

August 13, 2015

BlueskyReddit
Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD4 BV711

Catalog Number:

563050

I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. This CD4 stain with Brillian Violet 711 fluorochromes requires little antibody concentration and easily discriminates T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4 BV711

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry

Results Summary

Flow cytometric detection of CD4 works very well.

Additional Notes

None

Image Gallery

Summary

The Good

Good stain for CD4

The Bad

None to report

The Bottom Line

Provides good discrimination between T-cells and B-cell lymphocytes.

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