AbD Serotec
Goat Anti-Mouse Lambda Light Chain
106001
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Quantitative ELISAs for murine sera/mucosal samples require a murine standard. To detect an accurate standard, it is required to be bound to the ELISA plate. This antibody is one required to bind the murine antibody standard.
ELISA
Purified immunoglobulin
Overnight at 4C. Diluted 1:3200 in 1x PBS. Coated at 100ul per well.
1x PBS, 1% BSA, 0.05% Tween-20
Purified Ig diluted in 1x PBS, 1% BSA, 0.05% Tween-20 for 1hr at 37C
Goat anti-mouse Ig diluted in 1x PBS, 1% BSA, 0.05% Tween-20 for 1hr at 37C
Goat Anti-Mouse Immunoglobulin
This provides a standard, with an R2-value of more than 0.999 if used correctly, in concert with Goat Anti-mouse kappa light chain as the coating antibody.
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Availability, price, efficacy
A great, important antibody required for the coating for murine Immunoglobulin standards for use in a quantitative murine ELISA.