CD24 PerCP Cy5.5 Flow Cytometric Stain

August 18, 2015

BlueskyReddit
Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD24 PercP Cy5.5

Catalog Number:

562360

I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. The BD CD24 PerCP Cy5.5 flow stain reagent distinguished immature B-cells at final concentrations 1:2000.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

CD24 PerCP Cy5.5 flow cytometric stain works at 1:2000 and discriminates immature B-cells and was tested in splenocytes, blood and BM.

Additional Notes

None

Image Gallery

Summary

The Good

CD24 PerCP Cy5.5 flow cytometric stain provides great separation at 1:2000 and possibly even lower concentrations

The Bad

None

The Bottom Line

CD24 PerCP Cy5.5 flow stain provides a great immature B-cell stain with little optimization.

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