Invitrogen (Life Technologies)
Cell trace violet
C34557
We adoptively transfer T cells into recipient mice and then measure proliferation. Cell trace violet has been a great marker for proliferation. We use cell trace violet over CFSE because there is no bleed over into channels such as PE which are needed for other markers.
Flow Cytometry
Murine T cells
1:1000 for 20 minutes at 37 deg C in the dark
NA
Canto II flow cytometer
Great staining could still be detected even 33 days after initial staining and transfer into recipient animals. Clear cell divisions, no bleed over into other commonly used channels, less cell toxicity than what is seen with CFSE. In the sample image, antigen specific Thy1.1+ cells were stained with CTV, along with a Thy1.1neg CTV-stained antigen non-specific cotransfer which did not proliferate.
None
Great staining, minimal toxicity
Extra staining step because it works best at 37 deg C
Much better staining than CFSE.