Antibody to Analyze Renilla Luciferase Levels While Screen miRNA Inhibitors Using Dual Luciferase System

chemical biology
Indian institute of chemical technology
SRF

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Renilla luciferase

Catalog Number:

MAB4400

We are studying miRNA inhibitors in different cancers by using a dual luciferase system to find better therapeutic small molecules as the dual luciferase system is the best technical approach to screen miRNA inhibitors. We validate the dual luciferase system using these antibodies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549,MDA-MB,hela cell lysate

Primary Incubation

2hr at room temperature

Blocking Agent

Non-fat milk

Secondary Incubation

1hr at room temperature

Tertiary Incubation

NA

Detection

ECL from Amersham

Results Summary

Antibody works very well, easy to understand results and publishable quality

Additional Notes

If you keep it in the primary incubation for too long there is a chance it will become non-specifc .

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Summary

The Good

Affordable, and not bad

The Bad

A little non-specificty

The Bottom Line

Very good antibody and we can easily generate publishable quality blots.

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