Time Course Effect of Thrombin Induced ADRP Expression in Macrophages

August 10, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

Adipose differentiation related protein (ADRP)

Catalog Number:

sc-32888

Info: View Product View Product Specs

Browse Similar Products: ADRP Antibodies

Adipocyte differentiation-related protein (ADRP) is a lipid droplet associated protein. It expresses mostly in early adipose differentiation. It is present at the surface of the membrane and protects via intracellular lipids droplets. Here we checked the time course effect of thrombin on ADRP expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Cell lysate

Primary Incubation

1:500 in 5% BSA

Blocking Agent

5% Milk (blotting grade blocker) in 1XTBST

Secondary Incubation

1:4000 in 5% BSA

Tertiary Incubation

NA

Detection

GAR

Results Summary

Our results shows that there is an increased expression at 1min by thrombin.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

There is no/less background, the antibody is very efficient, and the signal is very good.

The Bad

A non-specific band comes just above the appropriate band which is a little bit confusing to identify.

The Bottom Line

This antibody is very good for Western blot since it does not give any background and the signal of this antibody is also very good. As I said above, a non-specific band was detected just above the appropriate band which is confusing. In my experience I would like to recommend to run a 12% gel rather than 10% for this particular antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion