CD19 BV786 Flow Cytometric Stain

August 14, 2015

BlueskyReddit
Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD19 BV711

Catalog Number:

563333

I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. This CD19 BV786 provides great B-cell discrimination.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

CD19 BV786 flow stain worked great at 1:200 concentration for 1-2 million cells per 100-200uL.

Additional Notes

None

Image Gallery

Summary

The Good

CD19 stain works great with splenocytes

The Bad

None

The Bottom Line

CD19 BV786 stain works great with splenocytes and other organ cells.

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