DNA Isolation From Plasma

August 10, 2015

IU
microbiology
student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Macherey-Nagel

Product Name:

Nucleospin plasma XS

Catalog Number:

740900.10

We were interested in identifying a specific virus from patient plasma samples. DNA isolation was needed to run PCR. This product was selected because of the low cost and being specific for isolation from plasma.

Experimental Design and Results Summary

Application

DNA isolation

Starting Material

Plasma

Protocol Overview

Followed manufacturer's instructions and eluted in 15-20 ul

Tips

Heat at end to help eliminate ethanol contamination

Results Summary

DNA was successfully isolated but had low purity

Additional Notes

None

Image Gallery

Summary

The Good

Quick protocol

The Bad

Low purity

The Bottom Line

Good product to use for DNA isolation from plasma

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