CD43 PE Flow Cytometric Stain

August 20, 2015

BlueskyReddit
Microbiology and Immunology
University of Miami
Post-doctorall fellow

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Company:

BD Biosciences

Product Name:

CD43 PE

Catalog Number:

553271

I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. This CD43 PE flow stain provides reasonable separation to be able to gate on pro B cells in the bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow cells

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Tested at 1:200 for 1 million cells in 200uL, CD43 PE stain gives enough separation to be able to gate on pro B cells in the bone marrow

Additional Notes

None

Image Gallery

Summary

The Good

CD43 PE stain provides reasonable separation

The Bad

Separation is not on par with other B-cell marker antibodies

The Bottom Line

This stain can be used in conjunction with other markers to be able to gate pro B cells

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