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Date Published
Easy Compensation of Amine-Reactive Dyes
(1)
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Thermo Fisher Scientific
March 16, 2016
Peritoneal leukocytes were being assessed for various markers and dead cells were excluded using an amine reactive dye (Tonbo Ghost 510). ...
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Time Course Effect of Chemoattractant on Gab1 Phosphorylation
(1)
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Cell Signaling Technology
March 15, 2016
GRB associated binder-1(Gab-1) is a adopter proteins which is playing major role in intercellular signaling cascades and activated by ...
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Clearly Distinguishes Mouse CD8b+ Peripheral Blood Leukocytes
(1)
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March 15, 2016
The goal of this experiment was to clearly distinguish CD8+ T cells in the peripheral blood of female C57Bl/6 mice for subsequent ...
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Best Ku80 Antibody
(1)
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GeneTex
March 15, 2016
Our lab uses Ku80 as a loading control for a number of our western blots, and we really need a clean antibody for those purposes. We tried ...
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APC Anti-Mouse CD8a Antibody
(1)
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BioLegend
March 15, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Real Time PCR Using SensiFAST SYBR Lo-ROX Kit
(1)
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Bioline
March 15, 2016
I have used SensiFAST SYBR Lo-ROX master mix for qPCR experiments to measure bacterial gene expressions. I have also compared the gene ...
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CO2 Incubator for Cell Culture
(1)
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Eppendorf
March 15, 2016
CO2 incubators are essential instruments for culturing various cell lines. Having a well designed and properly functioning incubator will ...
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CFX Connect Real-Time PCR Detection System
(1)
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Bio-Rad
March 15, 2016
We are assessing consequences of Aryl hydrocarbon Receptor (AhR) activation in dendritic cells. Specifically for these experiments we were ...
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Review of Anti-CD3 Alexa700
(1)
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March 15, 2016
The anti-CD3 antibody is routinely used as a pan-marker for T cells. Here is tested anti-CD3 conjugated to a relatively dim fluorochrome, ...
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PKA Kinase Activity Assay Kit
(1)
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Abcam
March 15, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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HDAC-1 Antibody
(1)
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MilliporeSigma
March 15, 2016
This product was used for Western blotting on infected or noninfected primary murine macrophages.
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Mini-PROTEAN System is Worth Every Penny
(1)
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Bio-Rad
March 14, 2016
It is slighly expensive but if you want to save lot of time just making gel waiting to be cast for an hrs, this is really a time saver.
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PE/Cy7 Anti-Mouse CD45 Antibody on Mouse BALF
(1)
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BioLegend
March 14, 2016
In this experiment, we need to identify bone marrow derived cells using CD45 as a marker following bacteria infection in mice. We selected ...
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MEK1 Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
March 14, 2016
This antibody was used to detect total MEK1 expression and used as a control for phosphorylated MEK1 at S221 (Novus Biologicals NB100-82033)...
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Inexpensive 7-AAD Viability Dye
(1)
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Tonbo Biosciences
March 14, 2016
Mice were injected IP with heat-killed E. coli and peritoneal lavage recovered. Immune populations were identified by flow cytometry on ...
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Great p19 Antibody
(1)
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Abcam
March 14, 2016
Our lab works with a number of different cell cycle regulator proteins in the context of several different types of cancer. We purchased ...
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Good Robot that Does Purification of Cells Conveniently
(1)
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March 14, 2016
Isolation of CD11b+ cells from multiple blood sample was desired. Robosep does it fast handling multiple samples at a time and with pre-...
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Relative Quantification of Bacterial Gene Expression
(1)
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Agilent Technologies
March 14, 2016
Expression of Escherichia coli genes responsible for acid resistance were measured in relative terms using Agilent's brilliant ultra-fast ...
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Measuring CD86 Expression
(1)
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BioLegend
March 14, 2016
This ab was used to measure CD86 maturation marker expression on mouse dendritic cells from the spleen.
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Bright, Inexpensive CD45 Antibody
(1)
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Tonbo Biosciences
March 14, 2016
Mice were interperitoneal injected with heat-killed E. coli and peritoneal lavage recovered. Immune populations were identified by flow ...
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The Antibody Stains IL-17A-Producing T-Cells Very Well
(1)
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March 14, 2016
We wanted to examine the proportion of CD4+ T-cells making IL-17A, so we used this antibody to distinguish the population of T-cells making ...
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Review of MagniSort™ Mouse CD4 T cell Enrichment Kit
(1)
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March 14, 2016
Isolation of CD4 or CD8 T cells by antibody + magnetic beads is a routine technique used in many immunology labs and there are several kits ...
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Review of Tim3 Pe-Cy7
(1)
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March 11, 2016
Tim3 is reported to be highly expressed on CD4 Th1 helper cells. Our goal was to evaluate the expression profile of Tim3 on splenocytes.
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EPOCH2: Excellent Plate Reader for Expert or Novice
(1)
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Agilent Technologies
March 11, 2016
The EPOCH2 plate reader gives the versatility and user-friendly software to develop just about any kind of assay (wavelength scan: 200-999nm...
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B-Actin Antibody Review
(1)
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Novus Biologicals, a Bio-Techne Brand
March 11, 2016
Infected or uninfected primary macrophages are western blotted for various proteins using b-Actin as a control.
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Automated Thawing Instrument
(1)
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BioCision, LLC
March 11, 2016
Most tissue culture experiment require cells. When using frozen cells, they need to be rapidly thawed at 37 degrees C. Traditionally, ...
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PE Anti-Mouse/Human CD11b Antibody on Mouse BALF Fluids
(1)
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BioLegend
March 11, 2016
PE anti-mouse/human CD11b Antibody from biolegend was used to identify lymphocytes CD11b populations from mouse infected with bacteria. We ...
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Anti-Mouse CD4 Antibody
(1)
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BioLegend
March 11, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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Time Course Effect of Chemoattractant on Pyk2 Phosphorylation
(1)
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Cell Signaling Technology
March 11, 2016
Pyk2 is a proline rich tyrosine kinase. It is one of the signaling mediators for the G protein coupled receptors and it is plays a important...
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PCNA Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
March 11, 2016
The goal of this experiment was to measure PCNA via western blot to assess cell proliferation in an H4IIE rat hepatoma cell line. We ...
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Human IL-1beta Cytokine Measurement
(1)
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Abcam
March 11, 2016
The general aim was to measure cytokine release, especially the release of IL-1beta in the BV2 stimulated cells. Stimulation is done to ...
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Staining of CD40 as an Activation Marker on Murine Splenocytes
(1)
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BioLegend
March 11, 2016
CD40 was stained on murine splenocytes to identify activated myeloid cells. Splenocytes were incubated with LPS over night to induce ...
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Awesome CD45 Antibody
(1)
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BioLegend
March 10, 2016
CD45 PEDazzle 594 was used as a new way to label HPCs in blood and various different tissues. We selected this product as it was a new color...
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Creating Gene Knockouts in E. coli
(1)
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New England Biolabs
March 10, 2016
Two PCR fragments flanking the region of desired gene to be deleted in E. coli were assembled into plasmid vector of 4 kb size using NEB ...
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Easy Kit to Determine IL-17A
(1)
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BioLegend
March 10, 2016
Mouse IL-17A produced in culture supernatants was detected using the pre-coated kit by following manufacturers instructions. Due to low ...
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Rat Leptin Estimation ELISA Kit
(1)
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R&D Systems, a Bio-Techne Brand
March 10, 2016
Leptin is a hormone produced by adipose cells that regulate energy balance by stopping hunger. Using the principle of sandwich ELISA, this ...
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GADD 34 detection via western blot
(1)
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Santa Cruz Biotechnology, Inc.
March 10, 2016
We tried this antibody as a measurement of ER stress in H4IIE rat hepatoma cell line. We treated cells with chemical or physiologic inducers...
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Review of Rat Anti-Mouse CD127 Brilliant Violet 510
(1)
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BD Biosciences
March 10, 2016
This antibody was used to detect IL-7Ra on T cells. This antibody conjugated to the BV510 fluorochrome was chosen because it was the only ...
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Nice FITC Anti-Mouse CD25 Antibody
(1)
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BioLegend
March 10, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Measurement of IL-8
(1)
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March 10, 2016
My project was related to sepsis and some possible inhibitors of sepsis related receptors. We wanted to check how my inhibitors are ...
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Phospho-MEK (Ser221) Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
March 10, 2016
This antibody was used to detect phosphorylation of MEK at S221 site in human breast cancer cell lines.
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Decent Vinvulin Antibody
(1)
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Cell Signaling Technology
March 10, 2016
Our lab uses Vinculin as a loading control in a number of our western blots, and we usually buy our antibodies from Cell Signaling because ...
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Perkin Elmer Lambda 35 Spectrophotometer
(1)
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March 09, 2016
This instrument has a wide range of wave lengths and a six cuvettes rack to absorb light in the sample. It is a good instrument for ...
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Good Anti-Rabbit HRP-Linked Secondary Antibody
(1)
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Cell Signaling Technology
March 09, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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Great Results with RSV Antibody
(1)
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Abcam
March 09, 2016
Anti- RSV N protein antibody was used for immunofluorescence. It worked great. We got very strong positive signal using green alexa ...
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Review of PD-L1 BV605
(1)
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BioLegend
March 09, 2016
This antibody was used in an experiment to identify the expression pattern of PD-L1 on splenic DC and macrophages.
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Anti-Human CD56-APC on Human Whole Blood
(1)
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BioLegend
March 09, 2016
The aim of this study was to identify human natural killer cells in whole blood.
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Good p53 Antibody
(1)
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Cell Signaling Technology
March 09, 2016
Like many other cancer labs, our lab works with p53. We recently needed to detect p53 protein levels in 786-0 cell lysates, and gave this ...
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First Strand cDNA Synthesis for qPCR
(1)
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Bioline
March 09, 2016
I have used Bioline's Tetro cDNA synthesis kit for synthesis of cDNA using random hexamers as primer with E. coli RNA samples to measure ...
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Clearly Distinguishes B220+ Peripheral Blood Leukocytes
(1)
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March 09, 2016
The goal of this experiment was to clearly distinguish B cells in the peripheral blood of female C57Bl/6 mice for subsequent phenotyping. I ...
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Incubator
Injector
Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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