EMD Millipore
HDAC-1 Antibody
06-720
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Browse Similar Products: HDAC-1 Western Blot Antibodies
This product was used for Western blotting on infected or noninfected primary murine macrophages.
Western Blot
Primary Macrophages
5% Milk 1 hour
5% Milk in TBST
1:1,000 Overnight at 4C
1:20,000 at RT for 45 Minutes
Developer
Optimization took a couple tries, but that could have been a result of the samples used for the experiment. Overall it's a good antibody for a histone deacetylase.
None
Reusable
Optimization was hard, not always clear bands
As far as specific HDAC antibodies go, this is a good one to use.