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Date Published
E. coli Genome Sequencing Using Nextera XT DNA Library Prep Kit
(1)
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Illumina
March 25, 2016
We routinely do bacterial isolates genome sequencing and for that we use Nextera XT DNA library prep kit. Usually we get very good quality ...
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HbA1c Analyzer
(1)
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March 25, 2016
Unmaintained diabetics are prone to elevated glycated haemoglobin levels where glucose binds with Hb protein. It is used to find out the ...
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Review of Fixable Viability Dye eFluor 506
(1)
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March 25, 2016
The Fixable Viability Dye eFluor 506 is a useful dye to distinguish live vs. dead cells prior fixable and permeablization. This dye can be ...
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Anti-Mouse/Rat IL-17A
(1)
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March 25, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Anti-TPP1 for Mouse Samples
(1)
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Abcam
March 25, 2016
We tested the levels of TPP1 in mouse lung to determine the role of TPP1 during oxidative stress.
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Purification of Ribosomal RNA Depleted Bacterial Total RNA
(1)
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QIAGEN - Sample to Insight
March 24, 2016
I have used RNeasy MinElute Cleanup Kit for purification of rRNA depleted gram negative total RNA. Recovery was almost 80% with total input....
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Eppendorf Has a Great ThermoMixer for All Applications
(1)
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Eppendorf
March 24, 2016
The focus of our lab is molecular imaging of cancer with antibody-based imaging agents. To do this, we must digest antibodies to obtain F(ab...
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Good Separation of CD4 Population
(1)
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Miltenyi Biotec
March 24, 2016
we analyzied CD 4 and CD8 population on lymph nodes . We chose milteny antibodies and got good results.
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Human CD3 (Brilliant Violet 785™)
(1)
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BioLegend
March 24, 2016
We are staining this antibody on whole cord blood in a 15-colour panel. It works very well to distinguish the T cell population.
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Anti-Mouse IL-4 antibody
(1)
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March 24, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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CD34 Antibody
(1)
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Biorbyt
March 24, 2016
This mAB is supposed to react with human, rat, cow, monkey and dog 34 in formalin-fixed tissues. It was tested against dog and horse bone ...
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Quantification of genomic DNA
(1)
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Thermo Fisher Scientific
March 24, 2016
I used Qubit dsDNA HS assay kit to quantify genomic DNA from bacterial and as well human cell lines. The kit was very specific to dsDNA and ...
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Time Course Effect of Chemoattractant on GSK-3ß Phosphorylation at Position Ser9
(1)
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Cell Signaling Technology
March 24, 2016
Glycogen Synthase kinase (GSK-3ß) is a ubiquitously expressed serine/threonine kinase that phosphorylates and inactivates glycogen synthase....
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Anti-ABCB11 Antibody
(1)
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BosterBio
March 23, 2016
We tried to use the Anti-ABCB11 antibody to stain bile canaliculi staining of sectioned spheroids. We did not have great success in our ...
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Review of Celltrace Far Red Cell Proliferation kit
(1)
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Thermo Fisher Scientific
March 23, 2016
This cell labeling kit was chosen as an alternative to CFSE and can be detected in the APC channel. The same protocol used with CFSE was ...
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This Generation of Beckman Coulter Microcentrifuges Could be Better
(1)
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March 23, 2016
In our research, we require microcentrifuges for spinning down samples, concentrating samples, and cleaning nanoparticle samples. As these ...
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Global microRNA Profiling
(1)
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Arraystar, Inc
March 23, 2016
The aim of this experiment was to perform global microRNA profiling on total RNA extracted from tissues. The total RNA samples were sent to ...
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PHAGOTEST™ Kit
(1)
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Novus Biologicals, a Bio-Techne Brand
March 23, 2016
The phagocytic function of leukocytes was compared in blood from healthy dogs or tumor-bearing dogs.
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Time Course Effect of Chemoattractant on GSK-3ß Tyrosine Phosphorylation
(1)
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BD Biosciences
March 23, 2016
Glycogen Synthase Kinase-3ß (GSK3ß) is a serine/threonine kinase which affects glycogen metabolism by phosphorylating various signaling ...
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The Antibody Stains CD14+ Small Intestinal Cells Well
(1)
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BD Biosciences
March 23, 2016
We wanted to examine the proportion of CD14+ cells in the small intestine in a diseased vs healthy state. To do so, we wanted to use flow ...
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Human CD3 (Brilliant Violet 650™)
(1)
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BioLegend
March 23, 2016
We are staining this antibody on whole cord blood in a 15-clolour panel. It works very well to distinguish the T cell population.
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ATM Antibody Review
(1)
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Cell Signaling Technology
March 23, 2016
This antibody was used for assessing ATM levels in bone marrow derived macrophages in virus infected mice.
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Anti-Mouse CD3 Antibody
(1)
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BioLegend
March 22, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Probe-Based qPCR Assay for Relative Gene Expression Analysis
(1)
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Integrated DNA Technologies
March 22, 2016
Expression of genes involved in bile stress in E. coli were analysed by performing probe based multiplexed qPCR assays using probes and ...
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Nice Separation of CD16+ Monocytes from Whole Blood
(1)
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BioLegend
March 22, 2016
This antibody was used in a pediatric study involving healthy and asthmatic patients. Multiple phenotyping panels were used to screen these ...
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Human CD4 (PE/Dazzle™ 594)
(1)
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BioLegend
March 22, 2016
We are staining this antibody on whole cord blood in a 15 colour panel. It works very well to detect Th cells.
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High Efficiency for Library Making
(1)
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Thermo Fisher Scientific
March 22, 2016
Stbl4 electro competent cells has high transformation efficiency that will help for the one who want to make complex library directly ...
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Bright F4/80 Antibody
(1)
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March 22, 2016
Peritoneal macrophages were identified by CD11b and F4/80.
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Total RNA Extraction from Whole Blood
(1)
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QIAGEN - Sample to Insight
March 22, 2016
Total RNA can be extracted from whole blood using the PAXgene Blood RNA kit. Whole blood is collected from an individual into a PAXgene ...
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Foxp3+ T Cells in the Spleen are Clearly Stained with the Antibody
(1)
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March 22, 2016
We wanted to determine the proportion of Treg cells in the spleen in a healthy versus a diseased state. To do this, we wanted to examine the...
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VE-Cadherin in Lung Microvessels
(1)
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Santa Cruz Biotechnology, Inc.
March 22, 2016
We determined expression of endothelial VE-cadherin in microvessels of intact blood-perfused lungs by in situ immunofluorescence. (Ref Am J...
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Quick and Easy Method for Western Blot Transfers
(1)
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Thermo Fisher Scientific
March 22, 2016
We are constantly running Western blots in our lab, so we wanted a fast and simple method for transferring protein from the gel to ...
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Anti-Human/Mouse CD44
(1)
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March 21, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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Time Course Effect of Chemoattractant Induced Ubiquitination in Mouse Cells
(1)
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Cell Signaling Technology
March 21, 2016
Ubiquitin is a highly conserved polypeptide and plays an important role in the proteosome pathway. Here we checked the time course effect of...
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Whole Blood Collection for Total RNA Extraction
(1)
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PreAnalytiX
March 21, 2016
PAXgene Blood RNA tubes contain a reagent that preserves the total RNA and prevents its degradation. This permits long term storage of ...
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Secondary FITC-Labeled Antibody for Flow Cytometry Could be Better
(1)
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SouthernBiotech
March 21, 2016
In our research, we need to examine the binding of antibodies to their corresponding cell surface receptor. In addition, we need to examine ...
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Total RNA Extraction from Gram Negative Bacteria
(1)
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QIAGEN - Sample to Insight
March 21, 2016
E. coli culture (3 ml) grown in LB medium to an optical density of 2.0 were collected in RNA protect bacteria reagent (6 ml) and pelleted ...
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Great Separation of B Cells
(1)
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BioLegend
March 21, 2016
This antibody was used within a phenotyping panel to screen patients' blood. This antibody offers a perfect separation of CD3- CD19+ HLA-DR...
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Human CD3 (Brilliant Violet 510™)
(1)
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BioLegend
March 21, 2016
We are using this antibody on full cordblood in a 15 colour panel. This antibody is good to distinguish the T cell population and gate the ...
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Synaptophysin Stains Retina Tissue
(1)
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March 21, 2016
We used Anti- Synaptophysin to stain synapses in mouse retina frozen and formalin fixed tissues.
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Review of Anti-V beta 5 FITC
(1)
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March 21, 2016
This antibody is routinely used to identify OT-II T cells. Here, I am using this antibody together with either anti-CD4 or anti-Va2 to ...
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CD204 Antibody
(1)
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Miltenyi Biotec
March 21, 2016
Macrophages were assessed for CD204 expression. Miltenyi's anti-CD204 was chosen because of the color options offered and bright, specific ...
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Review of CD62L PeCy7
(1)
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March 18, 2016
CD62L or L-selectin is routinely used in combination with CD44 to identify naive vs. memory T cells in the periphery. Here, I am using CD62L...
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Use of Vio Conjugated Antibody for Flow Cytometry Applications
(1)
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Miltenyi Biotec
March 18, 2016
Our research focuses on investigating bioactions of endogenous chemical mediators called resolvins that promote resolution of inflammation. ...
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Time Course Effect of Chemoattractant on ATF2 Phoshorylation
(1)
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Cell Signaling Technology
March 18, 2016
ATF2 is a transcription factor that regulates various genes. Here we checked the time course effect of ATF2 in response to a ...
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Anti-Mouse IL-9 Antibody
(1)
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BioLegend
March 18, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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One Compensation Bead for All Isotypes
(1)
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March 18, 2016
Compensation beads were used to determine spillover between fluorphores.
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Assay to Monitor Proteasome Dysfunction
(1)
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March 18, 2016
We wanted to test the proteasome function. So we used Proteasome Sensor Vector ZsProSensor-1 (Clontech #632425). ZsProSensor-1 is a ...
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Micro-Slides for Primary Cell Culture
(1)
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Ibidi GmbH
March 18, 2016
We are always on the lookout for fluorescent probes to stain cellular components in different primary cells or immortalized cell lines. ...
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Plasmid DNA Extraction from Gram Negative Bacteria
(1)
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QIAGEN - Sample to Insight
March 18, 2016
I have extracted plasmid DNA from E. coli cultures using QIAprep Spin Miniprep kit and got good quality plasmid DNA with yield of 9 ...
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Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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