B-Actin Antibody Review

March 11, 2016

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

B-Actin Antibody

Catalog Number:

NB600-532

Infected or uninfected primary macrophages are western blotted for various proteins using b-Actin as a control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary Macrophages

Primary Incubation

5% Milk 1 hour

Blocking Agent

Milk in TBST

Secondary Incubation

1:10,000 at 4C Overnight

Tertiary Incubation

1:20,000 at RT for 45 Minutes

Detection

Developer

Results Summary

B-Actin antibody produces robust and reliable staining for a variety of samples.

Additional Notes

None

Related Categories

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Summary

The Good

Can be reused multiple times

The Bad

Make sure it's diluted enough otherwise you will get overexposure

The Bottom Line

Great antibody, would highly recommend it.

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