eBioscience
Anti-mouse CD3 Alexa700
56-0032-82
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The anti-CD3 antibody is routinely used as a pan-marker for T cells. Here is tested anti-CD3 conjugated to a relatively dim fluorochrome, Alexa700.
Flow Cytometry
Splenocytes
30mins @ +4C
Anti-CD16/32
NA
LSR II
At 1:100 dilution, the anti-CD3 Alexa700 antibody gave a pretty poor separation between positive and background. I would recommend trying other antibodies for this dim fluorochrome.
None
Poor separation between positive and negative population
Best to try other antibodies conjugated to this dim fluorochrome.