Time Course Effect of Chemoattractant on Pyk2 Phosphorylation

March 11, 2016

BlueskyReddit
Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

Phospho-Pyk2 (Y402)

Catalog Number:

3291s

Pyk2 is a proline rich tyrosine kinase. It is one of the signaling mediators for the G protein coupled receptors and it plays an important role in cell spreading and migration. Here we checked its time course effect on chemoattractant induced Pyk2 phosphorylation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5%BSA prepared in 1xTBST

Blocking Agent

5% BSA prepared in 1xTBST

Secondary Incubation

1:4000 5%Milk prepared in 1XTBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found time dependent increase in Pyk2 phosphorylation by chemoattractant.

Additional Notes

None

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Summary

The Good

The antibody works effeciently and the results are accurate and easy to optmize.

The Bad

Generally this antibody gives good signal without any background noise but sometimes new lots gives more non-specific results.

The Bottom Line

Pyk2 tyrosine phosphorylation is involved in various signaling pathways. This antibody is suitable for western blot and it gives good signal. Highly recommended.

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