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Date Published
Anti-Mouse CD11c Antibody
(1)
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BioLegend
March 18, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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Fixable Viability Stain for Identification of Dead Cells
(1)
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March 18, 2016
This fixable viability stain was used to discriminate dead cells in primary murine tumor cells. Freshly isolated cells were stained with ...
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UltraComp: One Compensation Bead for All Isotypes
(1)
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March 17, 2016
These beads were used to determine spectral spillover for compensation.
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SYBR Green Master real time PCR
(1)
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Roche
March 17, 2016
SYBR Green Master mix is an ideal tool for real time PCR. It is easy to use, highly efficient, and much less expensive than ABI's similar ...
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Staining of CD80 as an Activation Marker on Murine Myeloid Cells
(1)
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BD Biosciences
March 17, 2016
CD80 was used to identify activated murine myeloid cells in spleen. Murine splenocytes were incubated with LPS over night to induce ...
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Anti-Mouse IL-6
(1)
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March 17, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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Purification of PCR Products
(1)
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QIAGEN - Sample to Insight
March 17, 2016
I have amplified a bacterial gene using PCR which was intended for cloning. I used QIA quick PCR purification kit to get rid of left over ...
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Anti-Mouse/Human CD11b Antibody
(1)
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BioLegend
March 17, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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PKC-theta Antibody
(1)
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Cell Signaling Technology
March 17, 2016
Protein Kinase C (PKC) are known to activate various cellular responses such as gene expression and proliferation. PKC? is predominently ...
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Total RNA & Protein Extraction from Peripheral Blood Mononuclear Cells
(1)
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March 17, 2016
The mirVana PARIS kit allows the extraction of total RNA, DNA as well as protein from the same sample. It was used in experiments where both...
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Review of Biotinylated Anti-Mouse Qa-2
(1)
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BD Biosciences
March 17, 2016
Qa-2 and CD24 are markers used to characterize recent thymic emigrants (RTE). Here I evaluated the expression pattern of Qa2 on T cells.
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Rhodopsin Primary Antibody
(1)
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Thermo Fisher Scientific
March 17, 2016
Rhodopsin, is a G-protein family which present in the photoreceptor layer where rods and cone cells are present.This protein detect the ...
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Good Antibody for Western Blotting
(1)
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BosterBio
March 16, 2016
This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background.
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Isotype Control for Canine IP (Brilliant Violet 650™ Mouse IgG2a, kappa)
(1)
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BioLegend
March 16, 2016
The main aim of this test was to phenotype dog blood cells and detect the presence or absence of lymphoma. Incubate whole blood cells with ...
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Phagoburst®
(1)
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ORPEGEN Pharma GmbH
March 16, 2016
The quantification of oxidative burst of peripheral blood mononuclear cells (PMN) was performed to compare respiratory burst function in ...
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Review of CD24 PeCy7
(1)
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March 16, 2016
CD24 or commonly known as heat stable antigen (HSA) is a marker routinely used to characterize thymocytes during their development in the ...
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Role of Intracellular Na2+ in Keratinocyte Proliferation/Maturation
(1)
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Abcam
March 16, 2016
It has already been reported that Na+/H+ exchanger is considerably increased during keratinocyte proliferation and migration. We wanted to ...
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Total RNA Extraction from Skin Biopsies
(1)
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March 16, 2016
The aim of this experiment was to extract total RNA from skin biopsies (approx. 4mm) for downstream microRNA quantification (qRT-PCR).
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HDAC-2 Antibody Review
(1)
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March 16, 2016
Infected or non-infected primary murine macrophages are western blotted for various proteins.
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Chemotaxis Slide for Suspension and Adherent Cells
(1)
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Ibidi GmbH
March 16, 2016
With this slide, we quantified chemotaxis of suspension cells (monocytes) using a chemoattractant. After acquiring images, the resulting ...
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Anti-Mouse CD19 Antibody
(1)
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March 16, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Easy Compensation of Amine-Reactive Dyes
(1)
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Thermo Fisher Scientific
March 16, 2016
Peritoneal leukocytes were being assessed for various markers and dead cells were excluded using an amine reactive dye (Tonbo Ghost 510). ...
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Time Course Effect of Chemoattractant on Gab1 Phosphorylation
(1)
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Cell Signaling Technology
March 15, 2016
GRB associated binder-1(Gab-1) is a adopter proteins which is playing major role in intercellular signaling cascades and activated by ...
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Clearly Distinguishes Mouse CD8b+ Peripheral Blood Leukocytes
(1)
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March 15, 2016
The goal of this experiment was to clearly distinguish CD8+ T cells in the peripheral blood of female C57Bl/6 mice for subsequent ...
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Best Ku80 Antibody
(1)
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GeneTex
March 15, 2016
Our lab uses Ku80 as a loading control for a number of our western blots, and we really need a clean antibody for those purposes. We tried ...
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APC Anti-Mouse CD8a Antibody
(1)
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BioLegend
March 15, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Real Time PCR Using SensiFAST SYBR Lo-ROX Kit
(1)
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Bioline
March 15, 2016
I have used SensiFAST SYBR Lo-ROX master mix for qPCR experiments to measure bacterial gene expressions. I have also compared the gene ...
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CO2 Incubator for Cell Culture
(1)
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Eppendorf
March 15, 2016
CO2 incubators are essential instruments for culturing various cell lines. Having a well designed and properly functioning incubator will ...
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CFX Connect Real-Time PCR Detection System
(1)
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Bio-Rad
March 15, 2016
We are assessing consequences of Aryl hydrocarbon Receptor (AhR) activation in dendritic cells. Specifically for these experiments we were ...
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Review of Anti-CD3 Alexa700
(1)
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March 15, 2016
The anti-CD3 antibody is routinely used as a pan-marker for T cells. Here is tested anti-CD3 conjugated to a relatively dim fluorochrome, ...
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PKA Kinase Activity Assay Kit
(1)
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Abcam
March 15, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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HDAC-1 Antibody
(1)
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MilliporeSigma
March 15, 2016
This product was used for Western blotting on infected or noninfected primary murine macrophages.
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Mini-PROTEAN System is Worth Every Penny
(1)
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Bio-Rad
March 14, 2016
It is slighly expensive but if you want to save lot of time just making gel waiting to be cast for an hrs, this is really a time saver.
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PE/Cy7 Anti-Mouse CD45 Antibody on Mouse BALF
(1)
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BioLegend
March 14, 2016
In this experiment, we need to identify bone marrow derived cells using CD45 as a marker following bacteria infection in mice. We selected ...
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MEK1 Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
March 14, 2016
This antibody was used to detect total MEK1 expression and used as a control for phosphorylated MEK1 at S221 (Novus Biologicals NB100-82033)...
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Inexpensive 7-AAD Viability Dye
(1)
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Tonbo Biosciences
March 14, 2016
Mice were injected IP with heat-killed E. coli and peritoneal lavage recovered. Immune populations were identified by flow cytometry on ...
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Great p19 Antibody
(1)
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Abcam
March 14, 2016
Our lab works with a number of different cell cycle regulator proteins in the context of several different types of cancer. We purchased ...
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Good Robot that Does Purification of Cells Conveniently
(1)
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March 14, 2016
Isolation of CD11b+ cells from multiple blood sample was desired. Robosep does it fast handling multiple samples at a time and with pre-...
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Measuring CD86 Expression
(1)
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BioLegend
March 14, 2016
This ab was used to measure CD86 maturation marker expression on mouse dendritic cells from the spleen.
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Relative Quantification of Bacterial Gene Expression
(1)
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Agilent Technologies
March 14, 2016
Expression of Escherichia coli genes responsible for acid resistance were measured in relative terms using Agilent's brilliant ultra-fast ...
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Bright, Inexpensive CD45 Antibody
(1)
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Tonbo Biosciences
March 14, 2016
Mice were interperitoneal injected with heat-killed E. coli and peritoneal lavage recovered. Immune populations were identified by flow ...
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The Antibody Stains IL-17A-Producing T-Cells Very Well
(1)
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March 14, 2016
We wanted to examine the proportion of CD4+ T-cells making IL-17A, so we used this antibody to distinguish the population of T-cells making ...
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Review of MagniSort™ Mouse CD4 T cell Enrichment Kit
(1)
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March 14, 2016
Isolation of CD4 or CD8 T cells by antibody + magnetic beads is a routine technique used in many immunology labs and there are several kits ...
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Review of Tim3 Pe-Cy7
(1)
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March 11, 2016
Tim3 is reported to be highly expressed on CD4 Th1 helper cells. Our goal was to evaluate the expression profile of Tim3 on splenocytes.
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EPOCH2: Excellent Plate Reader for Expert or Novice
(1)
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Agilent Technologies
March 11, 2016
The EPOCH2 plate reader gives the versatility and user-friendly software to develop just about any kind of assay (wavelength scan: 200-999nm...
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B-Actin Antibody Review
(1)
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Novus Biologicals, a Bio-Techne Brand
March 11, 2016
Infected or uninfected primary macrophages are western blotted for various proteins using b-Actin as a control.
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Automated Thawing Instrument
(1)
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BioCision, LLC
March 11, 2016
Most tissue culture experiment require cells. When using frozen cells, they need to be rapidly thawed at 37 degrees C. Traditionally, ...
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PE Anti-Mouse/Human CD11b Antibody on Mouse BALF Fluids
(1)
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BioLegend
March 11, 2016
PE anti-mouse/human CD11b Antibody from biolegend was used to identify lymphocytes CD11b populations from mouse infected with bacteria. We ...
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PCNA Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
March 11, 2016
The goal of this experiment was to measure PCNA via western blot to assess cell proliferation in an H4IIE rat hepatoma cell line. We ...
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Anti-Mouse CD4 Antibody
(1)
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BioLegend
March 11, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
Read Review
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