BioLegend
Mouse IL-17a detection kit
432507
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Mouse IL-17A produced in culture supernatants was detected using the pre-coated kit by following manufacturers instructions. Due to low levels this kit was preferred to minimize optimization etc.
ELISA
Culture supernatants
Protocol was followed as per BioLegend's insert that came along with the kit. Supernatant volume was between 35-50ul.
Not specific. Quantity of supernatant may be increased if not seeing any result.
Could detect pg quantity easily in treated vs non-treated samples
None
Easy to use
Expensive for one plate.
Overall good if fast determination needed from multiple samples accumulated over many experiments due to expense associated with it.
Product Name: LEGEND MAX™ Mouse IL-17A ELISA KitSupplier Page