Santa Cruz Biotechnology
GADD 34 (C-20)
sc-794
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Browse Similar Products: GADD Western Blot Antibodies
We tried this antibody as a measurement of ER stress in H4IIE rat hepatoma cell line. We treated cells with chemical or physiologic inducers of ER stress such as thapsigargin or the saturated fatty acid, palmitate and then probed for this antibody using western blot.
Western Blot
Total cell lysates of H4IIE rat hepatoma cells
1:100 in 1:3 Odyssey Block:TBST (0.1% Tween) overnight at 4 degrees C.
1:3 Odyssey Block:TBS for one hour at room temperature
1:15,000 Goat-anti rabbit in 1:3 Odyssey Block: TBST (0.1% Tween, 0.02% SDS) for one hour at room temperature
NA
Odyssey Infrared Imager
Picked up a whole lot of background. Cannot distinguish what band (if any) is GADD-34. Although I could have increased the antibody dilution to attempt to get rid of the background, I don't think anything would have showed up in the 73 kDA area.
None
No good
Lots of background. Can't detect the protein even with the lowest dilution.
This antibody is not worth the hassle.