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CD86 percp
105025
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A spleen was harvested from a C57BL/6 mouse and a single cell suspension was made. The cells were stained with the antibody that I am reviewing and also stained with an anti-mouse CD11c antibody. For data analysis, I gated on the CD11c positive population and assessed expression levels on a histogram for the antibody that I am reviewing. This ab was used to measure CD86 maturation marker expression on mouse dendritic cells from the spleen.
Flow Cytometry
Mouse splenocytes
1:200 on ice for 20min.
Fc Block
NA
Flow cytometry
The antibody stains intermediate and high CD86 expressors.
None
Can be easily titrated
Fluorochrome chosen may bleed over other channels
This antibody can stain cells brightly.