PE/Cy7 Anti-Mouse CD45 Antibody on Mouse BALF

Medicine
Harvard Medical school
Post doctoral research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD45 Antibody

Catalog Number:

103113

In this experiment, we need to identify bone marrow derived cells using CD45 as a marker following bacteria infection in mice. We selected this antibody because it is easy to use and we have used the APC/cy7 version of this product.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse BALF

Primary Incubation

1 in 200 20 mins

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Without much optimization we were able to identify cells that is stain cd45 positive from the BALF. The antibody can probably be further titrated.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use and affordable

The Bad

None

The Bottom Line

Very affordable antibody with good results

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