MEK1 Antibody from Novus Biologicals

March 14, 2016

BlueskyReddit
Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

MEK1 Antibody

Catalog Number:

NBP1-47833

This antibody was used to detect total MEK1 expression and used as a control for phosphorylated MEK1 at S221 (Novus Biologicals NB100-82033).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody perfectly detected total MEK1 expression by western blot in human breast cancer cell lines.

Additional Notes

None

Related Categories

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Summary

The Good

Signal is strong and specific.

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

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