Great Results with RSV Antibody

Infectious Diseases
St Jude Children's Research Hospital
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti- RSV nucleoprotein antibody

Catalog Number:

B023

Anti- RSV N protein antibody was used for immunofluorescence. It worked great. We got very strong positive signal using green alexa fluorophore as a secondary antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Vero cells infected with RSV A2 strain for 24 hours

Primary Incubation

1:500 dilution, incubation for 1 hour at room temperature

Blocking Agent

2% BSA solution blocking for 1 hour at room temperature

Secondary Incubation

Alexa Fluor 488 Goat anti mouse antibody at 1:1000 dilution for 1 hour at RT

Tertiary Incubation

Not needed

Detection

Fluorescent microscope

Results Summary

The antibody worked very well. Very strong positive signal in the RSV infected cells. Uninfected cells didn't show any activity.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very easy and quick to use

The Bad

Dilution factor

The Bottom Line

Highly recommend this product for detection of RSV N protein

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