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Date Published
Staining of CD40 as an Activation Marker on Murine Splenocytes
(1)
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BioLegend
March 11, 2016
CD40 was stained on murine splenocytes to identify activated myeloid cells. Splenocytes were incubated with LPS over night to induce ...
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Time Course Effect of Chemoattractant on Pyk2 Phosphorylation
(1)
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Cell Signaling Technology
March 11, 2016
Pyk2 is a proline rich tyrosine kinase. It is one of the signaling mediators for the G protein coupled receptors and it is plays a important...
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PCNA Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
March 11, 2016
The goal of this experiment was to measure PCNA via western blot to assess cell proliferation in an H4IIE rat hepatoma cell line. We ...
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Awesome CD45 Antibody
(1)
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BioLegend
March 10, 2016
CD45 PEDazzle 594 was used as a new way to label HPCs in blood and various different tissues. We selected this product as it was a new color...
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Creating Gene Knockouts in E. coli
(1)
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New England Biolabs
March 10, 2016
Two PCR fragments flanking the region of desired gene to be deleted in E. coli were assembled into plasmid vector of 4 kb size using NEB ...
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Easy Kit to Determine IL-17A
(1)
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BioLegend
March 10, 2016
Mouse IL-17A produced in culture supernatants was detected using the pre-coated kit by following manufacturers instructions. Due to low ...
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Rat Leptin Estimation ELISA Kit
(1)
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R&D Systems, a Bio-Techne Brand
March 10, 2016
Leptin is a hormone produced by adipose cells that regulate energy balance by stopping hunger. Using the principle of sandwich ELISA, this ...
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GADD 34 detection via western blot
(1)
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Santa Cruz Biotechnology, Inc.
March 10, 2016
We tried this antibody as a measurement of ER stress in H4IIE rat hepatoma cell line. We treated cells with chemical or physiologic inducers...
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Review of Rat Anti-Mouse CD127 Brilliant Violet 510
(1)
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BD Biosciences
March 10, 2016
This antibody was used to detect IL-7Ra on T cells. This antibody conjugated to the BV510 fluorochrome was chosen because it was the only ...
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Measurement of IL-8
(1)
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March 10, 2016
My project was related to sepsis and some possible inhibitors of sepsis related receptors. We wanted to check how my inhibitors are ...
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Nice FITC Anti-Mouse CD25 Antibody
(1)
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BioLegend
March 10, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Phospho-MEK (Ser221) Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
March 10, 2016
This antibody was used to detect phosphorylation of MEK at S221 site in human breast cancer cell lines.
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Decent Vinvulin Antibody
(1)
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Cell Signaling Technology
March 10, 2016
Our lab uses Vinculin as a loading control in a number of our western blots, and we usually buy our antibodies from Cell Signaling because ...
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Perkin Elmer Lambda 35 Spectrophotometer
(1)
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March 09, 2016
This instrument has a wide range of wave lengths and a six cuvettes rack to absorb light in the sample. It is a good instrument for ...
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Great Results with RSV Antibody
(1)
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Abcam
March 09, 2016
Anti- RSV N protein antibody was used for immunofluorescence. It worked great. We got very strong positive signal using green alexa ...
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Good Anti-Rabbit HRP-Linked Secondary Antibody
(1)
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Cell Signaling Technology
March 09, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-...
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Review of PD-L1 BV605
(1)
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BioLegend
March 09, 2016
This antibody was used in an experiment to identify the expression pattern of PD-L1 on splenic DC and macrophages.
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Anti-Human CD56-APC on Human Whole Blood
(1)
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BioLegend
March 09, 2016
The aim of this study was to identify human natural killer cells in whole blood.
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Good p53 Antibody
(1)
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Cell Signaling Technology
March 09, 2016
Like many other cancer labs, our lab works with p53. We recently needed to detect p53 protein levels in 786-0 cell lysates, and gave this ...
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First Strand cDNA Synthesis for qPCR
(1)
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Bioline
March 09, 2016
I have used Bioline's Tetro cDNA synthesis kit for synthesis of cDNA using random hexamers as primer with E. coli RNA samples to measure ...
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Clearly Distinguishes B220+ Peripheral Blood Leukocytes
(1)
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March 09, 2016
The goal of this experiment was to clearly distinguish B cells in the peripheral blood of female C57Bl/6 mice for subsequent phenotyping. I ...
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Western blot of CHOP as a measure of ER stress
(1)
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Cell Signaling Technology
March 09, 2016
We have tried this antibody multiple times in hopes it would work as a measure of ER stress chemical or physiological induction of ER stress...
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Antibody Anti Prolactin
(1)
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BosterBio
March 09, 2016
We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no ...
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Phospho-Akt (Thr 308) Antibody from Cell Signaling
(1)
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Cell Signaling Technology
March 08, 2016
This antibody was used to detect Akt phosphorylation at Thr 308 site in A431 cells
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Excellent Phospho p53 Antibody
(1)
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Cell Signaling Technology
March 08, 2016
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Pax6 Staining in Pancreatic Cryosection
(1)
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March 08, 2016
The Pax6 transcription factor is a pan-endocrine marker in the islets of langerhans. We were optimizing staining conditions in pancreatic ...
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Alpha Tubulin Santa Cruz Biotech
(1)
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Santa Cruz Biotechnology, Inc.
March 08, 2016
Wanted to use this antibody for western blot as well as fluorescent microscopy to assess the amount of total vs acetylated tubulin, each ...
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Best Phospho-JAK2 Antibody
(1)
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Cell Signaling Technology
March 08, 2016
Our lab works with a number of different JAK kinases in the context of kidney cancer, and we recently needed to blot for phospho-JAK2. We ...
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Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb Antibody Review
(1)
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Cell Signaling Technology
March 08, 2016
We are interested in studying a protein that we hypothesized to activate JNK when it is expressed in the cell. We transfected this protein ...
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Measurement of Sensitivity, Dynamic Range and Quantification of Differential Gene Expression
(1)
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Thermo Fisher Scientific
March 08, 2016
I have used ERCC ExFold RNA spike in mixes in my bacterial RNA seq library preparation to compare sensitivity, dynamic range and user ...
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Great Kit - Unattributed Review*
(1)
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BosterBio
March 08, 2016
I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve ...
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Time Course Effect of Chemoattractant on CD36 Expression
(1)
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Santa Cruz Biotechnology, Inc.
March 08, 2016
CD36 is a class B scavenger receptor which plays a major role in inflammation and atherosclerosis. Here we checked time course effect on ...
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FITC Anti-Mouse CD11c Antibody on Mouse Cells
(1)
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BioLegend
March 08, 2016
I want to identify lymphocyte populations in bacteria infected lung. We use the CD11c antibody as one of the markers for the lymphocyte ...
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Best Phospho-STAT3 Antibody
(1)
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Cell Signaling Technology
March 07, 2016
Our lab works with kinases involved in kidney cancer, including STAT3. We needed to reliably detect low protein levels of phosphorylated ...
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MTA2 Antibody from Bethyl Laboratories
(1)
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Bethyl Laboratories, Inc.
March 07, 2016
I am investigating functions of chromatin modification complexes in RNA expression. The goal of this experiment was to test the specificity ...
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Glut2 Staining in Pancreatic Cryosection
(1)
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Santa Cruz Biotechnology, Inc.
March 07, 2016
Glut2 is a low-affinity glucose transporter present in pancreatic beta cells, hepatocytes and basolateral membranes of intestinal and ...
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Anti-Hif-2a Antibody from Boster Bio
(1)
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BosterBio
March 07, 2016
We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other...
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Review of Anti-Mouse FR4 as a Treg Marker
(1)
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BD Biosciences
March 07, 2016
We wanted to sort live CD4 T regulatory cells for functional assays and one of the markers we used to identify Tregs is folate receptor 4 (...
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Clearly Shows Shift in CD154 Expression Among Peripheral Blood Leukocytes
(1)
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March 07, 2016
The goal of this experiment was to phenotype CD154 expression among leukocytes in the peripheral blood of female C57Bl/6 mice.
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PE/Cy7 Anti-Mouse/Human CD11b Antibody
(1)
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BioLegend
March 07, 2016
In order to study immune cell influx during a self limiting inflammatory response in the lung, LPS was instilled into mouse lungs. 24 hours ...
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ERK1/2 Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
March 07, 2016
This antibody was used to detect total expression of p44/42 MAPK (ERK1/2) in MDA-MB-231 human breast cancer cells and as a control for ...
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Bright Staining with HLA-DR Antibody
(1)
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BD Biosciences
March 07, 2016
This HLA-DR antibody was used to assess surface MHC expression on B cells.
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Digestion of Genomic DNA During RNA Extraction
(1)
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QIAGEN - Sample to Insight
March 07, 2016
I have used Qiagen RNase free DNase enzyme to digest genomic DNA during bacterial total RNA extraction. Successful removal of genomic DNA ...
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Review of Thy1.2 Alexa700
(1)
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BioLegend
March 04, 2016
This antibody conjugated to Alexa700 is routinely used in our lab to identify T cells and also in assessing T cell reconstitution in bone ...
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Anti-BDNF Antibody Performance
(1)
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BosterBio
March 04, 2016
This anti-BDNF antibody (Boster-PB9075) works great on the positive control every time, but we still have not received results for our ...
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Canine RF
(1)
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March 04, 2016
The immunoassay technique detects clinically significant levels of CRF. It is a rapid latex slide agglutination test kit that provides a ...
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Very Good Antibody for Mouse Ly6c
(1)
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BioLegend
March 04, 2016
I use this antibody in flow cytometry to study the distribution of ly6c marker in mouse organs under various conditions of inflammation.
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PE/Cy7 anti-mouse CD45
(1)
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BioLegend
March 04, 2016
Cells from brohchoalveolar lavage of LPS treated mice (24h post instillation, 5 micrograms) were stained for flow cytometry. CD45 antibody ...
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Best Phospho-AKT Antibody
(1)
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Cell Signaling Technology
March 04, 2016
Our lab works with all types of cancers, and we're often blotting for phospho-AKT to see downstream effects of mTOR manipulation. We gave ...
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Weakly Stains B220+ Peripheral Blood Leukocytes
(1)
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March 04, 2016
The goal of this experiment was to clearly distinguish B cells in the peripheral blood of female C57Bl/6 mice for subsequent phenotyping. I ...
Read Review
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Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
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Plate Reader
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Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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