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Date Published
Violet Laser Cell Tracking Dye
(1)
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Thermo Fisher Scientific
February 18, 2016
Thymocytes were stained for injection into the peritoneal cavity of mice. To be compatible with our previously designed flow panel a violet ...
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RBCS Surface Associated Immunoglobulin Antibody
(1)
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Bethyl Laboratories, Inc.
February 18, 2016
The aim of this experiment was to determine the proportion of RBC'S bound to IgM immunoglobulin from dogs that have Immune mediated ...
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CD140b Expression by Human Fibroblasts
(1)
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R&D Systems, a Bio-Techne Brand
February 18, 2016
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CD140b expression by human fibroblasts ...
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Great, Reliable ELISA
(1)
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R&D Systems, a Bio-Techne Brand
February 18, 2016
We analyzed murine lung homogenate supernatant, detection of KC was easy in infected samples.
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MIP-CreERT2 Pancreatic Cryosection 1 Day after Tamoxifen Treatment
(1)
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MilliporeSigma
February 18, 2016
Cre transgenes are driven by specific gene regulatory sequences, in our case, under the Mouse Insulin Promoter. The Cre-ERT2 is only ...
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Good Antibody to Detect Stromal Cells
(1)
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February 18, 2016
Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the ...
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CPSF160 Antibody from Bethyl Laboratories
(1)
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Bethyl Laboratories, Inc.
February 18, 2016
I am investigating functions of RNA 3'processing complexes. The goal of this experiment was to test the specificity of the antibody to ...
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CD62L Alexa Fluor 700 Antibody
(1)
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BioLegend
February 18, 2016
The general aim of my research is to investigate how lipids regulate immune cell function. The specific goal of this experiment is to ...
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Works in FACS, but Needs High Concentration
(1)
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BD Biosciences
February 18, 2016
This antibody was used on human PBMC cells stimulated in vitro with OKT3 for 2 and 3 days. For the optimization I used multiple ...
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Anti-ß-Actin Antibody from Sigma-Aldrich
(1)
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February 18, 2016
This antibody was used to detect expression of ß-Actin as a loading control for Western Blot.
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Differentiation of M1 Macrophages from Human PBMCs / Monocytes
(1)
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Miltenyi Biotec
February 17, 2016
Here, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, ...
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Great ELISA Kit for Measuring IGF1R Expression in Cells
(1)
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Abcam
February 17, 2016
Our lab deals with molecular imaging of cancer, but we must first know the expression of a specific target to choose the correct tumor-...
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LDHA Antibody from Cell Signaling
(1)
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Cell Signaling Technology
February 17, 2016
This antibody was used to detect LDHA expression in human cancer cell lines in response to cetuximab treatment.
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This Antibody Stains Foxp3+ T-Cells Very Well
(1)
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BD Biosciences
February 17, 2016
We wanted to examine proportions of Foxp3+ T-cells in healthy-state vs disease-state mice, so we used this antibody to distinguish Foxp3+ T-...
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Best PCR Purification Kit
(1)
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QIAGEN - Sample to Insight
February 17, 2016
Our lab performs a number of PCR reactions, and we often need to isolate the resulting amplified DNA from these reactions. The best way to ...
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Y2 Antibody Shows Strong Non-Specific Bands
(1)
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Biorbyt
February 17, 2016
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use western blots and subsequent...
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HLA Antibody
(1)
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BioLegend
February 17, 2016
This antibody was highly specific to the HLA antigens being produced in mammalian cell culture.
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Anti-Mouse PDL1
(1)
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BD Biosciences
February 17, 2016
This antibody was used on tumor mouse cell lines. It is a good anti-mouse PDL1 antibody - easy to optimize and gives small background.
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Bright, Inexpensive CD11b Antibody
(1)
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Tonbo Biosciences
February 17, 2016
CD11b and F480 were used to identify macrophages from naive and thioglycollate injected animals.
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Great Oligonuclutides at a Good Price
(1)
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Integrated DNA Technologies
February 17, 2016
I have had a good experience ordering primers and custom oligonucleuotides from IDT. They have always given me high-quality primers, shipped...
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CD276 Expression by Human Fibroblasts
(1)
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BioLegend
February 17, 2016
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CD276 expression by human fibroblasts ...
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Bright, Inexpensive Ly-6g Antibody
(1)
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February 16, 2016
Peritoneal leukocyte populations were being identified following heat-killed E. coli injection.
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Lactate Dehydrogenase (LDH) Activity Assay Kit from BioVision
(1)
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February 16, 2016
We used this kit for the measurement of LDH activity in human head and neck cancer cell lines in response to cetuximab treatment.
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Flow Cytometric Detection of Apoptosis (Chromatin Condensation)
(1)
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February 16, 2016
In this review we evaluate an easy-to-use flow-cytometry-based detection method for apotosis. We use this for a first screening of cell-...
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Great Analytical Balance
(1)
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February 16, 2016
Our lab masses out quantities of cancer drugs down to tenths of milligrams, and we need a good scale in order to do so. This particular ...
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Analog Vortex
(1)
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Fisher Scientific
February 16, 2016
This is used to mix samples and solutions made in the lab. It has variable speeds for different intensities of vortexing and has a couple ...
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Good Staining of CD4+ T Cells With V500 Antibody
(1)
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BD Biosciences
February 16, 2016
The aim of this study was a multicolor immunophenotypic analysis of peripheral blood of children. This antibody was selected because of its ...
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Good Marker for Sorting Naive CD4 T Cells
(1)
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BioLegend
February 16, 2016
We study T cell responses to antigen presenting cells. This is an excellent antibody for gating populations of CD4+ T cells in murine ...
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CXCL13 Expression by Human Fibroblasts
(1)
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R&D Systems, a Bio-Techne Brand
February 16, 2016
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CXCL13 expression by human fibroblasts ...
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Real Time PCR
(1)
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Agilent Technologies
February 16, 2016
Our study was aimed at determining the levels of message of different cytokines in variously treated tissues of mice. This machine is part ...
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CD273 Expression by Human Fibroblasts
(1)
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BioLegend
February 16, 2016
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CD273 expression by human fibroblast and...
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Complex Software for this Flow Cytometer
(1)
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BD Biosciences
February 16, 2016
Using the Facsdiva software is very complex--a lot you have to learn and get used to. Once all the parameters and tubes are set up on the ...
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ATP Bioluminescent Assay Kit from Sigma
(1)
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February 12, 2016
This kit was used to detect intracellular ATP levels in response to Cetuximab treatment.
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Mouse IL-1ß ELISA MAX™ Deluxe for the Detection IL-1ß Released from Microglia In Vitro
(1)
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BioLegend
February 12, 2016
Our aim was to monitor IL-1b release from cultured mouse microglia. For this we generated mixed glial cell cultures from neonatal mice (p0-2...
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Chemokine Response on Calcium Influx
(1)
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Thermo Fisher Scientific
February 12, 2016
We tested the time-course effect of a chemokine on calcium influx in human aortic smooth muscle cells.
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Isotype Control for K9 Immunophenotyping
(1)
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BioLegend
February 12, 2016
The purpse of the experiment was detection of B cell/T cell lymphoma using flow cytometry. We used this isotope control as a means to gate ...
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Macrophage Differentiation Using M-CSF
(1)
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Miltenyi Biotec
February 12, 2016
Our aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.
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ASMA Expression by Human Fibroblasts
(1)
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February 12, 2016
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the ASMA expression by human fibroblasts and...
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Good Specific Staining Antibody for gamma delta TCR
(1)
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BioLegend
February 12, 2016
Our experiment required depletion of BALB/c mice with the anti gamma delta TCR specific antibody in mice injected with a biological reagent....
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Good Mouse Rad51 Antibody
(1)
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BosterBio
February 12, 2016
The goal was to confirm the knockdown of mouse Rad51 via siRNA.
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Good Antibody to Boost the GFP Signal
(1)
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Thermo Fisher Scientific
February 12, 2016
We have used this antibody to enhance the GFP signal in some tissues form reporter mice that express GFP.
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Good Dye, Works to Stain Dead Cells
(1)
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February 12, 2016
This dye is good; it took a few optimization tries, but the dye works nicely. It is fixable and pretty consistent with results obtained with...
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TNFalpha Kit
(1)
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February 11, 2016
We wanted to check the cytokine production of our innate immune cells after stimulation. We used primary cells which are very sensitive, and...
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Ly6G Antibody Works great!
(1)
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Leinco Technologies, Inc.
February 11, 2016
This antibody was used to stain cells from murine bone marrow and murine lung (during infection) - looking at Ly6G to identify neutrophils.
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CD62L APC cy7 104427
(1)
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BioLegend
February 11, 2016
The general aim of my research is to investigate how lipids regulate immune cell function. The specific goal of this experiment is to ...
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CPSF30 Antibody from Bethyl Laboratories
(1)
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Bethyl Laboratories, Inc.
February 11, 2016
I am investigating functions of RNA 3'processing complexes. The goal of this experiment was to test the specificity of the antibody to ...
Read Review
Good Antibody to Detect Endothelial Cells
(1)
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February 11, 2016
Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the ...
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Homogenous Staining
(1)
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BioLegend
February 11, 2016
This was used to assess expression on dendritic cells in the mouse spleen.
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Time-Course Effect of Chemokine on PKC theta Phosphorylation in Human Aortic Smooth Muscle Cells
(1)
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Cell Signaling Technology
February 11, 2016
We tested the time-course effect of a chemokine on phosphorylation of PKCtheta in Human aortic smooth muscle cells.
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Podoplanin Expression by Human Fibroblasts
(1)
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BioLegend
February 11, 2016
Our work involves the characterization of human fibroblasts from different tissues. We analyzed Podoplanin expression by human fibroblasts ...
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