The Antibody Stains IL-17A-Producing T-Cells Very Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse/Rat IL-17A APC

Catalog Number:

17-7177-81

We wanted to examine the proportion of CD4+ T-cells making IL-17A, so we used this antibody to distinguish the population of T-cells making this cytokine after in vitro activation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of in vitro activated T-cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR II flow cytometer

Results Summary

The IL-17A-producing population of in vitro activated T-cells was very clearly distinguished using this antibody.

Additional Notes

Cells were fixed/permeabilized using the eBioscience Foxp3 staining kit

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Summary

The Good

Stains the IL-17A-producing T-cells very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identify IL-17A-producing T-cells

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