Best Ku80 Antibody

March 15, 2016

Research
University of North Carolina at Chapel Hill
Laboratory Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Genetex

Product Name:

Ku80 Antibody

Catalog Number:

GTX70485

Our lab uses Ku80 as a loading control for a number of our western blots, and we really need a clean antibody for those purposes. We tried this antibody out on a recommendation from another lab and have been pleased with the results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

786-0 Cell Lysates

Primary Incubation

1:1000 Dilution Overnight in 5% BSA in 1X TBS-T at 4C

Blocking Agent

5% Milk in 1X TBS-T

Secondary Incubation

1:5000 dilution of HRP-conjugated Rabbit secondary in 5% Milk in TBS-T for 1 hour at RT

Tertiary Incubation

None

Detection

Autoradiography Film using ECL alone

Results Summary

This antibody works great. It comes up with surprisingly soft bands, which is great for a loading control where you really need it to take up only a small area on your membranes. We needed a bit of optimization in terms of the dilution factor, but after we figured that out, it's been working great since.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use, comes up clean

The Bad

Optimization required

The Bottom Line

For the money, this is a great antibody. We've been using this antibody for years, and have no plans to change. I highly recommend this antibody for anyone that needs a good Ku80 loading control antibody.

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