Bright, Inexpensive CD45 Antibody

MBI
University of Rochester
Graduate Student

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Company:

Tonbo Biosciences

Product Name:

RedFluor™ 710 Anti-Mouse CD45

Catalog Number:

80-0451

Mice were interperitoneal injected with heat-killed E. coli and peritoneal lavage recovered. Immune populations were identified by flow cytometry on total peritoneal lavage. CD45 allows discrimination of leukocytes from debris and red blood cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Peritoneal cells - 8h after heat-killed E. coli injection

Primary Incubation

1:400 CD45-redFlour710

Blocking Agent

1:100 FcBlock - 5 minutes on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSRII Flow Cytometer

Results Summary

CD45 positive cells are clearly identified from debris and RBCs.

Additional Notes

None

Related Categories

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Summary

The Good

Inexpensive, bright staining

The Bad

None

The Bottom Line

An inexpensive antibody for identification of CD45 in AF700 channel.

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