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Coomassie SimplyBlue Stain
(1)
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Thermo Fisher Scientific
November 17, 2015
The purpose of this project was to separate soluble proteins from insoluble aggregates in rat liver cells. Before sending samples to ...
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Refrigerated Centrifuge
(1)
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November 17, 2015
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti-cancer immune therapies.
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STR-Testing Cell Line Authentication via Genetica DNA Labs
(1)
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November 17, 2015
Cell line contamination runs rampant among cell culture research, and often goes unchecked. Prominent research journals now require STR ...
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Boster IL-2 ELISA Kit
(1)
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BosterBio
November 17, 2015
I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA.
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Mounting Media with DAPI
(1)
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Thermo Fisher Scientific
November 17, 2015
This is used to mount immunostained cells onto slides. The product contains anti-fade to prolong fluorescence. It also contains DAPI, which ...
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Total s6 Ribosomal Protein
(1)
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Cell Signaling Technology
November 16, 2015
The general aim of this project was to assess protein synthesis in a variety of treatment groups using ribosomal protein s6 (rps6), a ...
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Good and Reliable Minispin Centrifuge
(1)
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Eppendorf
November 16, 2015
This mini centrifuge is very useful for the 1.5ml of tubes. It is a must-have tool when doing the RNA extraction process. This machine only ...
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Li-Cor 800cw Donkey Anti-Goat Antibody
(1)
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LICORbio
November 16, 2015
This is donkey anti-goat secondary antibody which we used a lot for western blot. It can be detected with the Li-Cor Oddysey machine at 800 ...
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Excellent Antibody for DAP12 Staining
(1)
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November 16, 2015
This product was used to identify intracellular DAP-12 expressing cells in various myeloid subsets.
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Fast RNA Purification from Trizol Reagent
(1)
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November 16, 2015
I used to directly FACS sort cells into Trizol reagent to keep the integrity of RNA. However, isolation of RNA from Trizol usually takes ...
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Anti-Mouse CD34 Antibody
(1)
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November 16, 2015
In this experiment we are looking at populations of immune cells in the bone marrow of mice.
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Great OSMR Primer/Probe Set
(1)
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Thermo Fisher Scientific
November 16, 2015
We use human sinus tissue, and this primer/probe set for OSMR is great.
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Cytochrome C Antibody Using Odyssey Detection
(1)
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Abcam
November 16, 2015
This antibody was used to detect mitochondria presence in rat liver. Total rat liver tissue was analyzed, as well as subcellular fractions ...
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ERK1/2 Antibody from Cell Signaling Technology
(1)
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Cell Signaling Technology
November 13, 2015
This antibody was used to detect total expression of p44/42 MAPK (ERK1/2) in response to cetuximab treatment in human HNSCC cells by western...
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Messy Staining for Class-II MHC
(1)
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MilliporeSigma, BioLegend
November 13, 2015
Class-II MHC is expressed on all antigen presenting cells including DCs, macrophages and B cells. The expression goes up during an immune ...
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CD44+ Staining of Murine Lymphocytes
(1)
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BD Biosciences
November 13, 2015
Following vaccination, the characteristics of the T cell responses induced are of importance. Differing vaccines, routes of immunisations, ...
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Labeling the Probe for Chemiluminescent EMSA
(1)
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November 13, 2015
This kit is used to label the probe which is used for chemiluminescent EMSA. Basically, it uses TdT to incorporate the 1-3 biotinylated ...
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Antibody to Detect B Cells
(1)
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BioLegend
November 13, 2015
CD19 is expressed on all pro-B to mature B cells (during development) and follicular dendritic cells, but not plasma cells; thus is an ...
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N2 Supplement in Serum-Free Neural Stem Cell Media for Culturing Glioma Caner Stem Cells
(1)
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Thermo Fisher Scientific
November 13, 2015
This product was used to culture glioma stem cells from glioblastoma cells (U87). The product supports glioma cell growth in serum-free ...
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Analysis of Expression of p53 in Activated CD8+ T Cells : A Time Course Study
(1)
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BioLegend
November 13, 2015
p53 is an important signaling molecule which has versatile roles in different stages of maturation and activation of a cell. We wanted to ...
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Detection of TNFa Secretion in Cells
(1)
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BioLegend
November 13, 2015
THP1 cells were differentiated into macrophage cells and treated with fraction 10 or control overnight. Cells were stimulated with LPS for 4...
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Enzyme immunoassay based Aflatoxin M1 detection kit
(1)
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r-biopharm
November 12, 2015
Milk samples were assayed for the presence of Aflatoxin M1.
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Good RNA Extraction Kit
(1)
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QIAGEN - Sample to Insight
November 12, 2015
This RNA extraction kit from Qiagen company is for the RNA extraction of up to 1X10^7 cells or less than 30mg of tissue. It works well and ...
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Quick and Good Kit to Assay Low IL-23 Levels
(1)
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BioLegend
November 12, 2015
This product was used to determine pg levels of IL-23 in in vivo models of autoimmune diseases. Because it's precoated, it's faster and I ...
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Anti-Mouse CD117 Antibody
(1)
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BD Biosciences
November 12, 2015
In this experiment we are looking at populations of immune cells in the bone marrow of mice.
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Akt1 Antibody from Cell Signaling
(1)
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Cell Signaling Technology
November 12, 2015
This antibody was used to detect total Akt1 expression and used as a control for phosphorylated Akt S473.
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Review of Mouse GITR-PeCy7
(1)
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BD Biosciences
November 12, 2015
This antibody gives a good detection of GITR, which is known to be expressed at a higher level in Foxp3+ Tregs than other T cell subsets. In...
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PD-1+ Staining of Murine Lymphocytes
(1)
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BioLegend
November 12, 2015
Following vaccination, exhaustion of the induced T cells is an important consideration. Exhaustion of the T cells can result in poor T cell ...
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Ponceau S Solution
(1)
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Santa Cruz Biotechnology, Inc.
November 11, 2015
Solution is used to detect protein bands on PVDF membranes to determine protein transfer efficiency in western blots. Easy to use solution ...
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Well-Engineered High-Quality Homogenizer
(1)
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Kinematica Inc.
November 11, 2015
For disruption of tissues or disruption of cells in culture when we want to minimize interfering chemicals, we use this mechanical ...
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Survivin Expression in Mouse T Cells
(1)
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Cell Signaling Technology
November 11, 2015
The aim of the research was to determine the levels of Survivin in unstimulated and stimulated mouse T cells. T cells were stimulated for ...
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Bright Stain for Interferon Gamma
(1)
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BD Biosciences
November 11, 2015
This antibody binds to mouse interferon gamma, a cytokine that is involved in T cell immunity and inflammation. Since, cytokines have a ...
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Mouse IL25 ELISA Kit
(1)
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November 11, 2015
I study release of various cytokines by immune cells in response to antigens in vitro as well as in vivo.
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Good Western Blot Antibody that Works
(1)
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MilliporeSigma
November 11, 2015
This antibody is mouse anti-human fibronectin N-terminal monoclonal antibody. Used in detecting human intervertebral disc tissue. Worked.
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T202/Y204-phosphorylated Erk Antibody from Cell Signaling Technology
(1)
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Cell Signaling Technology
November 11, 2015
This antibody is used to detect phosphorylation of Erk at T202/Y204 site in response to EGFR inhibitors in human HNSCC FaDu cells.
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Anti-Mouse CD115 Antibody
(1)
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BioLegend
November 11, 2015
In this experiment we were characterizing granulocytes in the blood of WT and KO mice.
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KDEL Antibody Used for Western Blot
(1)
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Abcam
November 11, 2015
This antibody was used to verify fraction purity in a subcellular fractionation of a rat liver experiment. KDEL was probed for in total ...
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CD4+ Staining of Murine Lymphocytes
(1)
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BD Biosciences
November 11, 2015
Following immunisation of novel vaccine candidates, it is of importance to measure the kinetics of antigen-specific T cells. An important ...
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Excellent GAPDH Antibody
(1)
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Santa Cruz Biotechnology, Inc.
November 10, 2015
I use GAPDH as a loading control for protein quantification by western blotting. It is one of the best antibodies for western blot. It works...
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Perhaps the Best Clone for Staining PD1
(1)
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November 10, 2015
PD1 (Programmed Death receptor 1) is expressed on chronically activated and exhausted T cells. Hence, it is only expressed during tumors or ...
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Very Good Anti-mouse TNF Antibody
(1)
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BD Biosciences
November 10, 2015
Splenocytes from C57BL/6 mice were stimulated for 6 hrs with PMA and Ionomycin in the presence of protein transport inhibitor Golgi Stop. ...
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Very Reliable Centrifuge
(1)
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Eppendorf
November 10, 2015
This Eppendorf bench centrifuge is very stable and reliable. It is the refrigerated style and works very well at temperature such as 4C. The...
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Good Anti-CD8 Antibody for FACS
(1)
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November 10, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-...
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pSTAT3 in Th17 Cells
(1)
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Cell Signaling Technology
November 10, 2015
The aim of my research was to determine the time course of IL-6-induced STAT3 phosphorylation in mouse Th17 cells.
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Drabkin’s Reagent for Hemoglobin Estimation
(1)
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November 10, 2015
Hemoglobin level in blood samples can be identified by Drabkin’s reagent.
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Detection of TNFa Secretion in Cells
(1)
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BioLegend
November 10, 2015
To analyze macrophage activation, THP1 cells were differentiated into macrophage cells and treated with fraction 10 or control overnight. ...
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Human TIMP-1 ELISA Kit
(1)
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Thermo Fisher Scientific
November 09, 2015
This kit is for the detection of human TIMP-1 protein concentration ELISA kit. It provided with every reagents needed and a pre-coated plate...
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Very Good Anti-Mouse IL-2 Antibody
(1)
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BD Biosciences
November 09, 2015
Mouse spleen cells were stimulated (6 hours) with PMA and Ionomycin in presence of protein transport inhibitor Golgi Stop. The cells were ...
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GCLM Antibody NBP1-33405 from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
November 09, 2015
This antibody is used to detect the expression of GCLM in different breast cancer cell lines.
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Use this Blocking Antibody in FACS Analysis
(1)
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BioLegend
November 09, 2015
To stain the neutrophil population in mouse peritoneal cells, mice were pretreated with a drug or PBS control, followed by injection with ...
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