Cell Signaling Technology
GCN5L2 (C26A10) Rabbit mAb
3305
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We were screening potential GCN5 knockout clones in mouse embryonic stem cells. We chose the antibody because the band on the image of Western blot on the antibody site looked specific and of the right size.
Western Blot
Mouse stem cells
1:1000 dilution overnight in 5% bsa at 4C
5% bsa
1:3000 antirabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
High background, band smaller than expected, not sure if it's specific.
None
There is a band.
Not sure if band is specific
Band is smaller than expected--not sure if it's specific.