IgD FITC Antibody Review

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

IgD FITC Antibody

Catalog Number:

405703

Splenocytes from latently infected mice are collected at various time points. Their immune cell populations are identified and quantified to compare the changes throughout latent infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:800, 20 minutes

Tertiary Incubation

NA

Detection

LSRII

Results Summary

B cell and T cell populations are identified on a broad spectrum as well as their subsets, and quantified. We had some trouble visualizing this antibody at first, but after optimizing realized that it was likely due to the cell population we were using.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Robust, Reliable

The Bad

None

The Bottom Line

Definitely recommend this antibody if this stain is needed for a panel

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