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FITC anti-mouse CD16/32
101305
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CD16/32 was used as a marker for endothelial cells in a CD45 negative population in the lung homogenate.
Flow Cytometry
Mouse lung tissue
1:200
NA
Flow cytometry
Mouse lungs were harvested, digested with collagenase and DNAse. Following digestions, cells were washed and pelleted. Cells were then stained for CD16/32 (1 in 200) for 20 mins. Cells were then washed and quantified through BD Canto II. The antibody is easy to use and the CD16/32 population separates easily.
None
Easy to use and clear signal
Very easy to optimize and easy to use