Excellent Antibody for Staining CD4+ T Cells

December 02, 2015

BlueskyReddit
Neurology
BWH
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP anti-mouse CD4

Catalog Number:

100538

Our laboratory is very interested in the role of regulatory T cells in autoimmunity. We mainly focus on regulatory CD4+FoxP3+ T cells and CD4+FoxP3-LAP+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen cells

Primary Incubation

20min 4'C, dilution 1/300 (50ul)

Blocking Agent

Fc block (anti CD16/CD32)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS LSRII

Results Summary

Bright and specific staining of CD4+ T cells.

Additional Notes

I like using PerCP for staining of CD4 T cells because CD4 is very highly expressed and thus it doesn't matter that PerCP is a pretty weak fluorochrome.

Image Gallery

Summary

The Good

Beautiful and reliable staining, no optimization required

The Bad

Nothing bad found

The Bottom Line

Perfect antibody for FACS staining of CD4+ T cells

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