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Protease and Phosphatase Inhibitor Cocktail
(1)
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October 21, 2015
This is used in western blot lysates to stop the degradation of proteins by proteases or removal of phosphate groups by phosphatases. It has...
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Capture Antibody for ELISA to Test Mouse TNF-alpha
(1)
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BioLegend
October 21, 2015
To test the anti-inflammatory effect of substances from bacteria fractions, we measured the secretion of TNFa in mouse macrophage cell lines...
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L (+) Arabinose for Bacteriological and Biochemical Purposes
(1)
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October 21, 2015
I studied disruption of ESAT6 and its natural binder CFP10 by another novel peptide, using bacterial three hybrid assay.
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Great Secondary Antibody for Visualizing Rat Primary antibodies in the AlexaFluor 594 Channel
(1)
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Thermo Fisher Scientific
October 21, 2015
When I stain tissue sections with a rat-derived primary antibody, I use this product to visualize the stain in the AlexaFluor 594 channel.
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Good Secondary Antibody
(1)
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Bio-Rad
October 21, 2015
I used this antibody for studying protein-protein interaction between ESAT6 and novel peptides identified using dot-blot western technique.
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Pierce BCA Protein Assay Kit from Thermo Scientific
(1)
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October 21, 2015
Quantitation of total protein in a sample is needed to be able to perform precise western blots. This kit was used to determine protein ...
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Works But Not as Well Built or Ergonomic as Competitors
(1)
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Hamilton Company
October 21, 2015
We use microplate-format assays for a number of procedures including routine protein quantification. A multichannel pipette helps and the ...
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Great for Staining/Visualizing Rabbit Primary Antibodies
(1)
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Thermo Fisher Scientific
October 20, 2015
I do a lot of immunofluorescent staining in lab, often using rabbit-derived primary antibodies to stain my antigen of interest. To visualize...
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Excellent Custom Oligonucleotide Service
(1)
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Integrated DNA Technologies
October 20, 2015
We study changes in gene expression using qPCR. Our primers are from IDT DNA because they have a clean website interface, useful tools for ...
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Homogenization and Genomic DNA Eliminator in 1 Column
(1)
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Omega Bio-Tek, Inc.
October 20, 2015
This is a homogenizer and genomic DNA eliminator column 2 in 1. It's the first step to do after lysing your cells; no more time-consuming ...
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Live/Dead Staining of Cells for Flow Cytometry
(1)
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Thermo Fisher Scientific
October 20, 2015
Live cells need to be distinguished from dead cells when running flow cytometry, and although DAPI can used in most cases for this ...
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The Best FACS Antibody for Phospho-STAT1 (Tyr701)
(1)
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Cell Signaling Technology
October 19, 2015
Our lab is interested in T cell signaling in autoimmunity and tolerance induction. This project aims at better understanding IL-27 signaling...
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Affordable Microcentrifuge Easily Fits Inside a Refrigerator
(1)
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October 19, 2015
I needed an affordable high speed microcentrifuge for a new lab. Units with built-in refrigeration cost a few thousand dollars. This unit is...
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Protease Inhibitor
(1)
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MilliporeSigma
October 19, 2015
This protease inhibitor cocktail is great for using to lyse cells to run western blots; it prevents protein degradation.
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Mouse IL-1 Protein Tried in Rabbit Disc Cell Culture
(1)
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BioLegend
October 19, 2015
This murine IL-1 protein was used to stimulate the cultured rabbit disc cell culture. Both human IL-1 and murine IL-1 protein were tried on ...
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Thrombin
(1)
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October 19, 2015
Thrombin is a serine protease which plays a major role in blood clotting and it is also one of the potent mitogens for major cell types.
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Serum-Free Neural Stem Cell Media for Culturing Glioma Cancer Stem Cells
(1)
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Thermo Fisher Scientific
October 19, 2015
We used this product to culture glioma stem cells from a U87 cell line. The product is free from Retinoic acid (which induces ...
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DAPI Stains Cell Nuclei Clearly
(1)
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Thermo Fisher Scientific
October 19, 2015
To visualize the general tissue structure and tissue organization during immunofluorescence staining, we use DAPI to stain the nuclei of all...
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Detecting Antibody for ELISA to Analyze Mouse TNFa
(1)
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BioLegend
October 19, 2015
We have isolated fractions from commensal bacteria secretions. To test if the secretions are able to suppress inflammatory activation, we ...
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Perfect for PCR Product Sequencing
(1)
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October 16, 2015
The goal of my this project is to identify the mutation in viral genome occurred during positive selection. Since I have tons of samples ...
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Good Isotype Control for APC Flow Antibody
(1)
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BioLegend
October 16, 2015
This isotype control was used for the isotype control of APC conjugated flow antibody. It worked well with double staining with PE color too...
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Bcl-xL Expression in Jurkat Cells
(1)
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Cell Signaling Technology
October 16, 2015
The aim of the research was to determine the levels of Bcl-xL in Jurkat cells after treatment with various cytokines. Jurkat cells were ...
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Great Secondary Antibody for Visualizing Rat Primary Antibodies in the AlexaFluor 488 Channel
(1)
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Thermo Fisher Scientific
October 16, 2015
When I stain tissue sections with a rat-derived primary antibody, I utilize this product to visualize the rat primary antibody stain in the ...
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B Cell Marker
(1)
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BioLegend
October 16, 2015
The aim of this experiment was to detect the cytokines in mouse B cells. This antibody works well to distinguish the B cell population.
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Ethidium Bromide Stains DNA Very Clearly
(1)
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Thermo Fisher Scientific
October 15, 2015
Our lab runs a lot of genotyping PCRs and, to visualize the PCR products, we often use ethidium bromide.
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The Best FACS Antibody for Phospho-STAT3 (Tyr705)
(1)
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Cell Signaling Technology
October 15, 2015
Our lab is interested in T cell signaling in the context of autoimmunity and tolerance induction. In this project I'm analyzing the effect ...
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Electrophoresis machine
(1)
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Bio-Rad
October 15, 2015
The power supply is good as you can save multiple programs and consistently works. You can also choose to set the voltage or amps constant. ...
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Good Antibody for Detecting GFP
(1)
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BioLegend
October 15, 2015
The aim of this experiment was to study the phosphorylation of Rb-analog, Whi5 in budding yeast after release from G1 arrest caused by ...
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Canine Immunophenotyping
(1)
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BioLegend
October 15, 2015
This product was used to perform immunophenotyping on dog samples for diagnosis of lymphoma. This isotope control was selected to serve as a...
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Excellent CD69 Antibody
(1)
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October 15, 2015
This antibody was to used to identify recently activated T cells and it worked very well.
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Extremely Strong Antibody Against V5 Epitope
(1)
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Thermo Fisher Scientific
October 15, 2015
The aim of this experiment was to study the phosphorylation of Rb-aanalog, Whi5 in budding yeast after release from G1 arrest caused by ...
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Premixed and Ready-To-Use Solution for DNA Synthesis
(1)
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Promega
October 14, 2015
GoTaq® Green Master Mix was mixed with primers which are specific for the gene of interest present in cDNA and kept in PCR machine for ...
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Great UV Vis Spectrophotometer for a Tight Budget
(1)
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VWR International
October 14, 2015
I needed an affordable UV Vis spec for a new lab to do basic quantification of proteins, nucleic acids, etc. Looking at various budget ...
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Best Available ATF2 Antibody for Immunofluorescence
(1)
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Cell Signaling Technology
October 14, 2015
Our aim was to identify mechanisms underlying ATF2 subcellular localization since nuclear localization is associated with poor prognosis in ...
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Good Antibody for Staining B Cells
(1)
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BioLegend
October 14, 2015
I used this antibody for staining B cells within lymphocytes. Compared to other fluorophores (BV510 etc.), this antibody showed much ...
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Excellent Antibody for Detecting Endogenous G6PDH
(1)
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October 14, 2015
The aim of this experiment was to study the expression dynamics of budding yeast G1 cyclin Cln2 after release from a G1 block caused by ...
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GFP Antibody
(1)
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Santa Cruz Biotechnology, Inc.
October 14, 2015
I transfected 293T cells with a vector having the GFP marker. To check the transfection after 72 hours, I performed western blot analysis. ...
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DNA Extraction
(1)
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Omega Bio-Tek, Inc.
October 13, 2015
We constantly have to sequence plasmid DNA in our lab. Before going to a maxi prep we always do a mini prep to send it for sequencing and ...
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Good Isotype Control for Flow
(1)
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BioLegend
October 13, 2015
This isotype control was ordered and applied as the isotype control for PE-conjugated flow antibody. It is easy to handle and result was ...
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Kit for cDNA Synthesis from RNA
(1)
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October 13, 2015
RNA isolated from liver tissue by TRIZOL method was used for the synthesis of cDNA by this High-Capacity cDNA Reverse Transcription Kit.
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Antibody Reacts With Mouse IgG2a Strongly
(1)
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Abcam
October 13, 2015
In my test result, Ab97245 reacts with mouse IgG2a heavy chain strongly. Ab97245 reacts with mouse IgG2a light chain slightly, which is the ...
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Standard Protein for Quantifying Mouse TNF-alpha
(1)
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BioLegend
October 13, 2015
We measured the secretion of TNFa in mouse macrophage cells after LPS stimulation in the presence or absence of the fractions/treatment. We ...
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Anti-Ki67 Stains Proliferating Cells in the Mouse Small Intestine
(1)
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Abcam
October 13, 2015
We were interested in examining the proliferation of the intestinal epithelial cells in healthy vs disease states. To do this, we stained ...
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Excellent Antibody for pErk1/2 Immunodetection in Mouse Tissue
(1)
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Cell Signaling Technology
October 13, 2015
Antibody was tested for detection of phospho- p44/42 MAPK (Erk1/2) in mouse tissue protein lysate. Mouse tissue samples were collected in ...
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Good and Easy to Use Antibody
(1)
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BioLegend
October 12, 2015
This antibody was used to detect the CCR2 expression on the human leukemia cell line THP cell line. Cells were stained with this antibody ...
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Western Blot Analysis of Phospho-EGFR (Y1173)
(1)
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Thermo Fisher Scientific
October 12, 2015
This product was used to test the phosphorylation of EGFR after treatment with EGF.
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Sensitive and Quantitative Detection for Mouse Anti-Nuclear Antibodies
(1)
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Alpha Diagnostic International Inc
October 12, 2015
We are interested in detecting anti-nuclear antigen autoantibodies (autoimmune predisposition) in aged mouse serum and comparing that with ...
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Excellent p300 CT Antibody
(1)
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MilliporeSigma
October 12, 2015
In the present study, mammalian cells were tested for p300 prorein levels in response to treatment. Equal amount of proteins were resolved ...
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ETF Maxi Kit
(1)
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QIAGEN - Sample to Insight
October 12, 2015
Using the ETF Maxi kit from Qiagen allows fast and efficient isolation of plasmid DNA suitable for transfection and cloning. In as little ...
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Proliferation Assay for Flow Cytometry Using Edu
(1)
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October 12, 2015
We set out to investigate the proliferative properties of U87 Glioblastoma cell lines. Since we were not convinced using BrdU Assays because...
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