RNA Miniprep Kit

Harvard
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

QIAGEN

Product Name:

RNeasy Mini Kit

Catalog Number:

74104

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Application

RNA purification and clean up

Starting Material

Cells and Tissues

Protocol Overview

Cells were lysed, added with ethanol and loaded onto the RNeasy silica membrane Mini spin columns. After washing, bound RNA was eluted in 30–50 µl nuclease free water.

Tips

Always treat the working area with DEPC water

Results Summary

High quality pure RNAs were extracted from mammalian cells for qPCR and cloning experiments

Additional Notes

None

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Summary

The Good

Very efficient and easy to use RNA miniprep kit

The Bad

None

The Bottom Line

Pure, concentrated RNA is eluted every time, ready to use for qPCR and cloning experiments.

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