Good Ready Made Staining Solution

University of Illinois, Chicago
Department of Microbiology and Immunology
PostDoctoral Research Fellow

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Company:

Bio-Rad

Product Name:

Coomassie Brilliant Blue R-250 Staining Solution

Catalog Number:

1610436

Info: Supplier Page 

Coomassie brilliant blue solution is required for staining the protein bands after SDS-PAGE. I use the ready made solution from Bio-Rad and I am really satisfied with the staining it gives. It is highly specific and sensitive. There is no blue background post-destaining, which makes every gel a great looking gel.

Experimental Design and Results Summary

Application

SDS-PAGE

Starting Material

Purified protein samples

Protocol Overview

I loaded different proteins samples in the gel and ran SDS-PAGE. I stained the gel with coomassie brilliant blue solution for 30 minutes and destained it for another 30 minutes.

Tips

Always handle it carefully, it has methanol and acetic acid.

Results Summary

Bands were clean, sharp and bright.

Additional Notes

None

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Summary

The Good

Highly specific, sharp staining

The Bad

None

The Bottom Line

It's an amazing reagent and can be reused several times.

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